Anti-hnRNP M3-M4 antibody [2A6-2H3] (ab128143)
Key features and details
- Mouse monoclonal [2A6-2H3] to hnRNP M3-M4
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG2b
Overview
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Product name
Anti-hnRNP M3-M4 antibody [2A6-2H3]
See all hnRNP M3-M4 primary antibodies -
Description
Mouse monoclonal [2A6-2H3] to hnRNP M3-M4 -
Host species
Mouse -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Full length native Human proteins hnRNP M3-M4 (NP_005959.2, NP_112480.2)
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Positive control
- 293T cell lysate, HeLa cell lysate, Jurkat cell lysate, NIH 3T3 cell lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. The product can be stored for up to 12 months. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 99% Tris buffered saline, 0.1% BSA
pH 7 to 8 -
Concentration information loading...
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Purity
IgG fraction -
Purification notes
ab128143 was produced via Mouse Hybridoma. The hybridoma was grown in a bioreactor and the antibody was purified using a mouse immunoglobulin binding ligand. -
Clonality
Monoclonal -
Clone number
2A6-2H3 -
Isotype
IgG2b -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab128143 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 77 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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Notes |
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WB
1/2000 - 1/10000. Predicted molecular weight: 77 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
Target
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Function
Pre-mRNA binding protein in vivo, binds avidly to poly(G) and poly(U) RNA homopolymers in vitro. Involved in splicing. Acts as a receptor for carcinoembryonic antigen in Kupffer cells, may initiate a series of signaling events leading to tyrosine phosphorylation of proteins and induction of IL-1 alpha, IL-6, IL-10 and tumor necrosis factor alpha cytokines. -
Sequence similarities
Contains 3 RRM (RNA recognition motif) domains. -
Post-translational
modificationsSumoylated. -
Cellular localization
Nucleus, nucleolus. - Information by UniProt
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Database links
- Entrez Gene: 4670 Human
- Entrez Gene: 76936 Mouse
- Omim: 160994 Human
- SwissProt: P52272 Human
- SwissProt: Q9D0E1 Mouse
- Unigene: 465808 Human
- Unigene: 666214 Human
- Unigene: 311439 Mouse
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Alternative names
- CEA receptor antibody
- CEAR antibody
- Heterogeneous nuclear ribonucleoprotein M antibody
see all
Images
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All lanes : Anti-hnRNP M3-M4 antibody [2A6-2H3] (ab128143) at 0.04 µg/ml
Lane 1 : 293T cell lysate at 50 µg
Lane 2 : 293T cell lysate at 15 µg
Lane 3 : HeLa cell lysate at 50 µg
Lane 4 : Jurkat cell lysate at 50 µg
Lane 5 : NIH 3T3 cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 77 kDa
Exposure time: 30 seconds -
Immunoprecipitation : 1 mg of 293T cell lysate immunoprecipitated with ab128143 at 3 µg/mg lysate; 20% of immunoprecipitate loaded in lane
Western Blot: ab128143 at 0.04 µg/ml
developed using the ECL technique
Exposure time : 30 seconds
Predicted band size : 77 kDa
Protocols
Datasheets and documents
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Datasheet download
References (0)
ab128143 has not yet been referenced specifically in any publications.