RabMAb

Anti-HNF-4-alpha antibody [EPR3648] (ab92378)

Overview

  • Product nameAnti-HNF-4-alpha antibody [EPR3648]
    See all HNF-4-alpha primary antibodies
  • Description
    Rabbit monoclonal [EPR3648] to HNF-4-alpha
  • Tested applicationsWB, IP, IHC-P, ICC, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Human

    Does not react with

    Mouse, Rat
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human HNF-4-alpha aa 1-100.

  • Positive control
    • HepG2, A549 and SW480 cell lysates, Human colon tissue, Human kidney tissue
  • General notes

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    This product is available conjugated to DyLight® 488 see ab139906.

    A 40 μl trial size is available to purchase for this antibody.

Applications

Our Abpromise guarantee covers the use of ab92378 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 53 kDa.
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
ICC 1/100 - 1/250.
Flow Cyt 1/100.
ICC/IF 1/100.

Target

  • FunctionTranscriptionally controlled transcription factor. Binds to DNA sites required for the transcription of alpha 1-antitrypsin, apolipoprotein CIII, transthyretin genes and HNF1-alpha. May be essential for development of the liver, kidney and intestine.
  • Involvement in diseaseDefects in HNF4A are the cause of maturity-onset diabetes of the young type 1 (MODY1) [MIM:125850]; also symbolized MODY-1. MODY is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease.
  • Sequence similaritiesBelongs to the nuclear hormone receptor family. NR2 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • Post-translational
    modifications
    Phosphorylated on tyrosine residue(s); phosphorylation is important for its DNA-binding activity. Phosphorylation may directly or indirectly play a regulatory role in the subnuclear distribution.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ39654 antibody
    • Hepatic nuclear factor 4 alpha antibody
    • Hepatocyte nuclear factor 4 alpha antibody
    • Hepatocyte nuclear factor 4 antibody
    • Hepatocyte nuclear factor 4-alpha antibody
    • HNF 4 alpha antibody
    • HNF 4 antibody
    • HNF-4-alpha antibody
    • HNF4 antibody
    • HNF4A antibody
    • HNF4A_HUMAN antibody
    • HNF4a7 antibody
    • HNF4a8 antibody
    • HNF4a9 antibody
    • Hnf4alpha antibody
    • HNF4alpha10/11/12 antibody
    • MODY 1 antibody
    • MODY antibody
    • MODY1 antibody
    • NR2A1 antibody
    • NR2A21 antibody
    • Nuclear receptor subfamily 2 group A member 1 antibody
    • OTTHUMP00000031060 antibody
    • OTTHUMP00000031062 antibody
    • TCF 14 antibody
    • TCF antibody
    • TCF-14 antibody
    • TCF14 antibody
    • Tcf4 antibody
    • Transcription factor 14, hepatic nuclear factor antibody
    • Transcription factor 14 antibody
    • Transcription factor HNF 4 antibody
    • Transcription factor HNF-4 antibody
    • Transcription factor HNF4 antibody
    see all

Anti-HNF-4-alpha antibody [EPR3648] images

  • All lanes : Anti-HNF-4-alpha antibody [EPR3648] (ab92378) at 1/1000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : A549 cell lysate
    Lane 3 : SW480 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size : 53 kDa
  • ab92378 staining HNF-4-alpha in Formalin-fixed, Paraffin-embedded Human colon tissue (A) and Human kidney tissue (B) using ab92378 at 1/100 dilution. Detection: DAB staining

  • ab92378 at 1/100 dilution staining HNF-4-alpha in HepG2 cells by Immunofluorescence.

  • Overlay histogram showing HepG2 cells stained with ab92378 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab92378, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

References for Anti-HNF-4-alpha antibody [EPR3648] (ab92378)

ab92378 has not yet been referenced specifically in any publications.

Product Wall

Application Flow Cytometry
Fixation Paraformaldehyde
Permeabilization Yes - Permeabilization Buffer
Sample Human Cell (Differentiated Cardiac Ventricular Cells)
Specification Differentiated Cardiac Ventricular Cells
Gating Strategy Isotype negative control (white)
Preparation Cell harvesting/tissue preparation method: Typsin-EDTA Cell Dissociation
Sample buffer: PBS and 10% FBS
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Abcam user community

Verified customer

Submitted Jan 29 2014

Our antigen retrieval method used for ab92378 and ab129189 is described below.

Antigen Retrieval

This is recommended Heat Induced Epitope Retrieval (HIER) using Decloaking Chamber/Pressure Cooker. Hot water bath or Microwave with te...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 37°C
Sample Human Cell (HepG2)
Specification HepG2
Permeabilization Yes - BD Perm/Wash Buffer
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted May 22 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (HepG2)
Specification HepG2
Preparation Cell harvesting/tissue preparation method: Purchased in as frozen cell suspension
Sample buffer: DMSO freezing mix
Fixation Paraformaldehyde
Permeabilization Yes - Perm/Wash buffer
Gating Strategy Using isotype control as a negative population, everything else was gated for Vinculin
Username

Abcam user community

Verified customer

Submitted May 14 2013

Thank you fo ryour message.

To clarify regarding ab135618 Anti-PAX8 antibody, the datasheet states we sell this at 100 ug, at a concentration of 0.25 mg/ml. Therefore, you can calculate that at this concentrations, 100 ug will be in 250 ul o...

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Thank you for your message which has been forwarded to the scientific support team.

I am sorry it is difficult to let you know the amount of antibody required for one analysis. This will be very individual and will depend on:

1. T...

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Thank you for contacting us. Antibodies are viable for 1 year at 4C and 10 years at -20C. Please check the datasheet of individual antibody for storage instructions. I hope this information is helpful to you. Please do not hesita...

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A BLAST search shows that ab92378 can detect isoforms 1 and 7. This antibody can also detect isoforms 2, 4, and 8. Sequence alignment shows that its immunogen shares 100% identity with isoforms 3 and 9. Overall, it is predicted to react with all isofor...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"