Anti-HNF-4-alpha + HNF-4-gamma antibody (ab64911)

Overview

  • Product nameAnti-HNF-4-alpha + HNF-4-gamma antibody
  • Description
    Rabbit polyclonal to HNF-4-alpha + HNF-4-gamma
  • SpecificityRabbit polyclonal to HNF-4-alpha + HNF-4-gamma antibody detects endogenous levels of total HNF-4-alpha/gamma protein.
  • Tested applicationsSuitable for: ICC/IF, ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human HNF-4-alpha + HNF-4-gamma (internal sequence).

  • Positive control
    • Extracts from HeLa cells, HepG2 cells and 293 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab64911 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
ELISA 1/20000.
WB 1/500 - 1/1000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).

Anti-HNF-4-alpha + HNF-4-gamma antibody images

  • All lanes : Anti-HNF-4-alpha + HNF-4-gamma antibody (ab64911) at 1/500 dilution

    Lane 1 : extracts from HeLa cells
    Lane 2 : extracts from HepG2 cells
    Lane 3 : extracts from 293 cells
    Lane 4 : extracts from 293 cells with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 52 kDa
    Observed band size : 52 kDa
    Additional bands at : 30 kDa,40 kDa,65 kDa,75 kDa,90 kDa. We are unsure as to the identity of these extra bands.
  • ICC/IF image of ab64911 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab64911, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-HNF-4-alpha + HNF-4-gamma antibody (ab64911)

ab64911 has not yet been referenced specifically in any publications.

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