Anti-HNF-4-alpha (phospho S313) antibody (ab78356)

Overview

  • Product nameAnti-HNF-4-alpha (phospho S313) antibody
    See all HNF-4-alpha primary antibodies
  • Description
    Rabbit polyclonal to HNF-4-alpha (phospho S313)
  • SpecificityThis antibody detects endogenous levels of HNF-4-alpha only when phosphorylated at serine 313.
  • Tested applicationsSuitable for: WB, ELISA, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human HNF-4-alpha. Synthesized phosphopeptide derived from human HNF-4-alpha around the phosphorylation site of serine 313 (L-R-SP-Q-V).

  • Positive control
    • Extracts from HUVEC cells. Human liver carcinoma tissue. LOVO cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab78356 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 54 kDa).
ELISA 1/20000.
IHC-P 1/50 - 1/100.
ICC/IF 1/500 - 1/1000.

Target

  • FunctionTranscriptionally controlled transcription factor. Binds to DNA sites required for the transcription of alpha 1-antitrypsin, apolipoprotein CIII, transthyretin genes and HNF1-alpha. May be essential for development of the liver, kidney and intestine.
  • Involvement in diseaseDefects in HNF4A are the cause of maturity-onset diabetes of the young type 1 (MODY1) [MIM:125850]; also symbolized MODY-1. MODY is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease.
  • Sequence similaritiesBelongs to the nuclear hormone receptor family. NR2 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • Post-translational
    modifications
    Phosphorylated on tyrosine residue(s); phosphorylation is important for its DNA-binding activity. Phosphorylation may directly or indirectly play a regulatory role in the subnuclear distribution.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ39654 antibody
    • FRTS4 antibody
    • Hepatic nuclear factor 4 alpha antibody
    • Hepatocyte nuclear factor 4 alpha antibody
    • Hepatocyte nuclear factor 4 antibody
    • Hepatocyte nuclear factor 4-alpha antibody
    • HNF 4 alpha antibody
    • HNF 4 antibody
    • HNF-4-alpha antibody
    • HNF4 antibody
    • HNF4A antibody
    • HNF4A_HUMAN antibody
    • HNF4a7 antibody
    • HNF4a8 antibody
    • HNF4a9 antibody
    • Hnf4alpha antibody
    • HNF4alpha10/11/12 antibody
    • MODY 1 antibody
    • MODY antibody
    • MODY1 antibody
    • NR2A1 antibody
    • NR2A21 antibody
    • Nuclear receptor subfamily 2 group A member 1 antibody
    • OTTHUMP00000031060 antibody
    • OTTHUMP00000031062 antibody
    • TCF 14 antibody
    • TCF antibody
    • TCF-14 antibody
    • TCF14 antibody
    • Tcf4 antibody
    • Transcription factor 14, hepatic nuclear factor antibody
    • Transcription factor 14 antibody
    • Transcription factor HNF 4 antibody
    • Transcription factor HNF-4 antibody
    • Transcription factor HNF4 antibody
    see all

Anti-HNF-4-alpha (phospho S313) antibody images

  • All lanes : Anti-HNF-4-alpha (phospho S313) antibody (ab78356) at 1/500 dilution

    Lane 1 : Extracts from HUVEC cells, treated with EGF (200ng/ml, 30mins)
    Lane 2 : Extracts from HUVEC cells, treated with EGF (200ng/ml, 30mins) with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 54 kDa
    Observed band size : 54 kDa
  • Immunohistochemistry analysis of paraffin-embedded human liver carcinoma tissue using ab78356 at a 1/50 dilution.
    Left image un-treated.
    Right image treated with immunizing peptide.
  • Immunofluorescence analysis of LOVO cells using ab78356 at a 1/500 dilution.
    Left image un-treated.
    Right image treated with immunizing peptide.

References for Anti-HNF-4-alpha (phospho S313) antibody (ab78356)

ab78356 has not yet been referenced specifically in any publications.

Product Wall


The most common way to detect a primary antibody in western blotting is to use a secondary antibody conjugated to a reporter enzyme, such as horseradish peroxidase. For example, a secondary antibody you could use with ab78356 is ab16284. For compl...

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