Anti-HNF-4-alpha (phospho S313) antibody (ab78356)


  • Product nameAnti-HNF-4-alpha (phospho S313) antibody
    See all HNF-4-alpha primary antibodies
  • Description
    Rabbit polyclonal to HNF-4-alpha (phospho S313)
  • SpecificityThis antibody detects endogenous levels of HNF-4-alpha only when phosphorylated at serine 313.
  • Tested applicationsSuitable for: WB, ELISA, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human HNF-4-alpha. Synthesized phosphopeptide derived from human HNF-4-alpha around the phosphorylation site of serine 313 (L-R-SP-Q-V).

  • Positive control
    • Extracts from HUVEC cells. Human liver carcinoma tissue. LOVO cells.



Our Abpromise guarantee covers the use of ab78356 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 54 kDa).
ELISA 1/20000.
IHC-P 1/50 - 1/100.
ICC/IF 1/500 - 1/1000.


  • FunctionTranscriptionally controlled transcription factor. Binds to DNA sites required for the transcription of alpha 1-antitrypsin, apolipoprotein CIII, transthyretin genes and HNF1-alpha. May be essential for development of the liver, kidney and intestine.
  • Involvement in diseaseDefects in HNF4A are the cause of maturity-onset diabetes of the young type 1 (MODY1) [MIM:125850]; also symbolized MODY-1. MODY is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease.
  • Sequence similaritiesBelongs to the nuclear hormone receptor family. NR2 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • Post-translational
    Phosphorylated on tyrosine residue(s); phosphorylation is important for its DNA-binding activity. Phosphorylation may directly or indirectly play a regulatory role in the subnuclear distribution.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ39654 antibody
    • FRTS4 antibody
    • Hepatic nuclear factor 4 alpha antibody
    • Hepatocyte nuclear factor 4 alpha antibody
    • Hepatocyte nuclear factor 4 antibody
    • Hepatocyte nuclear factor 4-alpha antibody
    • HNF 4 alpha antibody
    • HNF 4 antibody
    • HNF-4-alpha antibody
    • HNF4 antibody
    • HNF4A antibody
    • HNF4A_HUMAN antibody
    • HNF4a7 antibody
    • HNF4a8 antibody
    • HNF4a9 antibody
    • Hnf4alpha antibody
    • HNF4alpha10/11/12 antibody
    • MODY 1 antibody
    • MODY antibody
    • MODY1 antibody
    • NR2A1 antibody
    • NR2A21 antibody
    • Nuclear receptor subfamily 2 group A member 1 antibody
    • OTTHUMP00000031060 antibody
    • OTTHUMP00000031062 antibody
    • TCF 14 antibody
    • TCF antibody
    • TCF-14 antibody
    • TCF14 antibody
    • Tcf4 antibody
    • Transcription factor 14, hepatic nuclear factor antibody
    • Transcription factor 14 antibody
    • Transcription factor HNF 4 antibody
    • Transcription factor HNF-4 antibody
    • Transcription factor HNF4 antibody
    see all

Anti-HNF-4-alpha (phospho S313) antibody images

  • All lanes : Anti-HNF-4-alpha (phospho S313) antibody (ab78356) at 1/500 dilution

    Lane 1 : Extracts from HUVEC cells, treated with EGF (200ng/ml, 30mins)
    Lane 2 : Extracts from HUVEC cells, treated with EGF (200ng/ml, 30mins) with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.

    Predicted band size : 54 kDa
    Observed band size : 54 kDa
  • Immunohistochemistry analysis of paraffin-embedded human liver carcinoma tissue using ab78356 at a 1/50 dilution.
    Left image un-treated.
    Right image treated with immunizing peptide.
  • Immunofluorescence analysis of LOVO cells using ab78356 at a 1/500 dilution.
    Left image un-treated.
    Right image treated with immunizing peptide.

References for Anti-HNF-4-alpha (phospho S313) antibody (ab78356)

ab78356 has not yet been referenced specifically in any publications.

Product Wall

The most common way to detect a primary antibody in western blotting is to use a secondary antibody conjugated to a reporter enzyme, such as horseradish peroxidase. For example, a secondary antibody you could use with ab78356 is ab16284. For compl...

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