Anti-HNF1 alpha antibody (ab96777)
Key features and details
- Rabbit polyclonal to HNF1 alpha
- Suitable for: IP, WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-HNF1 alpha antibody
See all HNF1 alpha primary antibodies -
Description
Rabbit polyclonal to HNF1 alpha -
Host species
Rabbit -
Tested applications
Suitable for: IP, WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Pig -
Immunogen
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HepG2 whole cell lysate IHC-P: A549 Xenograft
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Purified by antigen-affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab96777 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP |
Use at an assay dependent concentration.
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WB | (1) |
1/500 - 1/3000. Detects a band of approximately 81 kDa (predicted molecular weight: 67 kDa).
Lysates should be made freshly and used in WB immediately to minimize protein degradation. |
IHC-P |
1/100 - 1/250.
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ICC/IF |
1/100 - 1/1000.
Recommend using PFA fixation instead of Methanol fixation. |
Notes |
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IP
Use at an assay dependent concentration. |
WB
1/500 - 1/3000. Detects a band of approximately 81 kDa (predicted molecular weight: 67 kDa). Lysates should be made freshly and used in WB immediately to minimize protein degradation. |
IHC-P
1/100 - 1/250. |
ICC/IF
1/100 - 1/1000. Recommend using PFA fixation instead of Methanol fixation. |
Target
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Function
Transcriptional activator that regulates the tissue specific expression of multiple genes, especially in pancreatic islet cells and in liver. Required for the expression of several liver specific genes. Binds to the inverted palindrome 5'-GTTAATNATTAAC-3'. -
Tissue specificity
Liver. -
Involvement in disease
Defects in HNF1A are a cause of hepatic adenomas familial (HEPAF) [MIM:142330]. Hepatic adenomas are rare benign liver tumors of presumable epithelial origin that develop in an otherwise normal liver. Hepatic adenomas may be single or multiple. They consist of sheets of well-differentiated hepatocytes that contain fat and glycogen and can produce bile. Bile ducts or portal areas are absent. Kupffer cells, if present, are reduced in number and are non-functional. Conditions associated with adenomas are insulin-dependent diabetes mellitus and glycogen storage diseases (types 1 and 3). Note=Bi-allelic inactivation of HNF1A, whether sporadic or associated with MODY3, may be an early step in the developmant of some hepatocellular carcinomas.
Defects in HNF1A are the cause of maturity-onset diabetes of the young type 3 (MODY3) [MIM:600496]; also symbolized MODY-3. MODY is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease.
Defects in HNF1A are the cause of susceptibility to diabetes mellitus insulin-dependent type 20 (IDDM20) [MIM:612520]. IDDM20 is a multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These features can result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels. -
Sequence similarities
Belongs to the HNF1 homeobox family.
Contains 1 homeobox DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 503578 Cow
- Entrez Gene: 6927 Human
- Entrez Gene: 21405 Mouse
- Entrez Gene: 574067 Pig
- Entrez Gene: 24817 Rat
- Omim: 142410 Human
- SwissProt: P20823 Human
- SwissProt: P22361 Mouse
see all -
Alternative names
- Albumin proximal factor antibody
- Hepatic nuclear factor 1 alpha antibody
- Hepatic nuclear factor 1 antibody
see all
Images
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Anti-HNF1 alpha antibody (ab96777) at 1/5000 dilution + HepG2 whole cell lysate at 30 µg
Predicted band size: 67 kDa
Observed band size: 81 kDa why is the actual band size different from the predicted?7.5% SDS PAGE
Blocking: 5% non-fat milk in TBST, room temperature, 60 minutes.
Lysates should be made freshly and used in WB immediately to minimize protein degradation. The molecular weight observed is consistent with what has been described in the literature (PMID:31145732).
Negative control: HeLa (PMID: 12488960). Also, 293T and A431.
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ab96777, at a 1/100 dilution, staining HNF1 alpha in paraffin embedded A549 Xenograft by Immunohistochemistry.
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ab96777 staining HNF1 alpha in HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% paraformaldehyde at room temperature for 15 minutes. Samples were incubated with primary antibody (1/1000) at 4°C overnight and stained green, alpha tubulin stained red and Hoecsht 33342 stained blue.
Permeabilization: 0.1% Triton X-100 in PBS, 4 minutes.
Blocking: 2.5% BSA/PBS for 30 minutes.
Secondary antibody: Rabbit IgG antibody (Alexa Fluor 488), 1:2000 (keep from light), room temperature for 1 hour.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (14)
ab96777 has been referenced in 14 publications.
- Low BSJ et al. Decreased GLUT2 and glucose uptake contribute to insulin secretion defects in MODY3/HNF1A hiPSC-derived mutant ß cells. Nat Commun 12:3133 (2021). PubMed: 34035238
- Hu M et al. Loss of HNF1a Function Contributes to Hepatocyte Proliferation and Abnormal Cholesterol Metabolism via Downregulating miR-122: A Novel Mechanism of MODY3. Diabetes Metab Syndr Obes 13:627-639 (2020). PubMed: 32184642
- Cheng S et al. Romidepsin (FK228) in a Mouse Model of Lipopolysaccharide-Induced Acute Kidney Injury is Associated with Down-Regulation of the CYP2E1 Gene. Med Sci Monit 26:e918528 (2020). PubMed: 31954012
- Ma P et al. HNF1A-Induced lncRNA HCG18 Facilitates Gastric Cancer Progression by Upregulating DNAJB12 via miR-152-3p. Onco Targets Ther 13:7641-7652 (2020). PubMed: 32801777
- Sun QJ et al. The Role of Bone Morphogenetic Protein 9 in Nonalcoholic Fatty Liver Disease in Mice. Front Pharmacol 11:605967 (2020). PubMed: 33603666