Overview

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab83289 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 51 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay, 1:312500.

Target

Anti-HNF6 antibody images



  • Predicted band size : 51 kDa
    Western blot analysis of Human fetal lung tissue lysate labeling HNF6 with ab83289 at 1.0µg/ml.


  • Predicted band size : 51 kDa
    Western blot analysis of Human fetal liver tissue lysate labeling HNF6 with ab83289 at 1.0µg/ml.


  • Predicted band size : 51 kDa
    Western blot analysis of Human fetal heart tissue lysate labeling HNF6 with ab83289 at 1.0µg/ml.
  • Anti-HNF6 antibody (ab83289) at 1 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 51 kDa
    Observed band size : 51 kDa
    Additional bands at : <22 kDa. We are unsure as to the identity of these extra bands.

References for Anti-HNF6 antibody (ab83289)

ab83289 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"