Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab83564 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 51 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use a concentration of 0.2 - 1 µg/ml.

Target

Images

  • Anti-HNF6 antibody (ab83564) at 1 µg/ml (in 5% skim milk / PBS buffer) + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 51 kDa
    Observed band size : 51 kDa
    12% gel
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human brain, cerebellum tissue labelling HNF6 with ab83564 at 5.0µg/ml.

References

This product has been referenced in:
  • Yuan XW  et al. Hepatocyte Nuclear Factor 6 Suppresses the Migration and Invasive Growth of Lung Cancer Cells through p53 and the Inhibition of Epithelial-Mesenchymal Transition. J Biol Chem 288:31206-16 (2013). WB ; Human . Read more (PubMed: 24022481) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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