Immunocytochemistry/ Immunofluorescence - Anti-HNMT antibody - Aminoterminal end (ab63147)
ICC/IF image of ab63147 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab63147, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Western blot - HNMT antibody - Aminoterminal end (ab63147)
Anti-HNMT antibody - Aminoterminal end (ab63147) at 1/50 dilution + Hela cell line lysates at 35 µg
Immunohistochemical analysis of HNMT expression in paraffin embedded formalin fixed human hepatocarcinoma tissue using 1/10 ab63147, followed by peroxidase-conjugated secondary antibody step and DAB staining.
References for Anti-HNMT antibody - Aminoterminal end (ab63147)
This product has been referenced in:
Stegaev V et al. Histamine transport and metabolism are deranged in salivary glands in Sjogren's syndrome. Rheumatology (Oxford)N/A:N/A (2013).
Read more (PubMed: 23709238) »
Szabó PM et al. Differences in the expression of histamine-related genes and proteins in normal human adrenal cortex and adrenocortical tumors. Virchows Arch455:133-42 (2009).
Read more (PubMed: 19568768) »