Overview

  • Product nameAnti-HNMT antibody - Aminoterminal end
    See all HNMT primary antibodies
  • Description
    Rabbit polyclonal to HNMT - Aminoterminal end
  • Tested applicationsSuitable for: ICC/IF, WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (Human) conjugated to KLH of the N terminal region of human HNMT.

  • Positive control
    • Hela cell line lysates and human hepatocarcinoma tissue. IF/ICC: MCF7 cell line.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab63147 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB 1/50 - 1/100. Detects a band of approximately 36 kDa (predicted molecular weight: 33 kDa).
ELISA 1/1000.
IHC-P 1/10 - 1/50.

Target

  • FunctionInactivates histamine by N-methylation. Plays an important role in degrading histamine and in regulating the airway response to histamine.
  • Sequence similaritiesBelongs to the methyltransferase superfamily. HNMT family.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • EC 2.1.1.8 antibody
    • Histamine N methyltransferase antibody
    • Histamine N-methyltransferase antibody
    • HMT antibody
    • HNMT antibody
    • HNMT S1 antibody
    • HNMT S2 antibody
    • HNMT_HUMAN antibody
    see all

Anti-HNMT antibody - Aminoterminal end images

  • ICC/IF image of ab63147 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab63147, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-HNMT antibody - Aminoterminal end (ab63147) at 1/50 dilution + Hela cell line lysates at 35 µg

    Predicted band size : 33 kDa
    Observed band size : 36 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemical analysis of HNMT expression in paraffin embedded formalin fixed human hepatocarcinoma tissue using 1/10 ab63147, followed by peroxidase-conjugated secondary antibody step and DAB staining.

References for Anti-HNMT antibody - Aminoterminal end (ab63147)

This product has been referenced in:
  • Stegaev V  et al. Histamine transport and metabolism are deranged in salivary glands in Sjogren's syndrome. Rheumatology (Oxford) N/A:N/A (2013). Read more (PubMed: 23709238) »
  • Szabó PM  et al. Differences in the expression of histamine-related genes and proteins in normal human adrenal cortex and adrenocortical tumors. Virchows Arch 455:133-42 (2009). IHC-P ; Human . Read more (PubMed: 19568768) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"