For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
Synthetic peptide (Human) conjugated to KLH of the N terminal region of human HNMT.
Our Abpromise guarantee covers the use of ab63147 in the following tested applications.
|ICC/IF||Use a concentration of 5 µg/ml.|
|WB||1/50 - 1/100. Detects a band of approximately 36 kDa (predicted molecular weight: 33 kDa).|
|IHC-P||1/10 - 1/50.|
ICC/IF image of ab63147 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab63147, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.