Overview

  • Product nameAnti-hnRNP A1 antibody
    See all hnRNP A1 primary antibodies
  • Description
    Rabbit polyclonal to hnRNP A1
  • Tested applicationsSuitable for: ICC/IF, WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog, Pig, Chimpanzee
  • Immunogen

    A region within synthetic peptide: MSKSESPKEP EQLRKLFIGG LSFETTDESL RSHFEQWGTL TDCVVMRDPN, corresponding to N terminal amino acids 1-50 of Human hnRNP A1

  • Positive control
    • WB: Jurkat cell lysate. IHC-P: Hepatocytes from human liver. IHC-P: Myocardial cells from human heart.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab50492 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 39 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.
IHC-P Use a concentration of 4 - 8 µg/ml.

Target

  • FunctionInvolved in the packaging of pre-mRNA into hnRNP particles, transport of poly(A) mRNA from the nucleus to the cytoplasm and may modulate splice site selection. May play a role in HCV RNA replication.
  • Sequence similaritiesContains 2 RRM (RNA recognition motif) domains.
  • Post-translational
    modifications
    Arg-194, Arg-206 and Arg-225 are dimethylated, probably to asymmetric dimethylarginine.
    Sumoylated.
  • Cellular localizationNucleus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Shuttles continuously between the nucleus and the cytoplasm along with mRNA. Component of ribonucleosomes. In the course of viral infection, colocalizes with HCV NS5B at speckles in the cytoplasm in a HCV-replication dependent manner.
  • Information by UniProt
  • Database links
  • Alternative names
    • HNRNPA 1 antibody
    • Helix destabilizing protein antibody
    • Helix-destabilizing protein antibody
    • Heterogeneous nuclear ribonucleoprotein A1 antibody
    • Heterogeneous nuclear ribonucleoprotein A1B protein antibody
    • Heterogeneous nuclear ribonucleoprotein B2 protein antibody
    • Heterogeneous nuclear ribonucleoprotein core protein A1 antibody
    • hnRNP A1 antibody
    • hnRNP core protein A1 antibody
    • HNRNPA1 antibody
    • HNRPA1 antibody
    • MGC102835 antibody
    • Nuclear ribonucleoprotein particle A1 protein antibody
    • ROA1_HUMAN antibody
    • Single strand DNA binding protein UP1 antibody
    • Single strand RNA binding protein antibody
    • Single-strand RNA-binding protein antibody
    see all

Anti-hnRNP A1 antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human heart tissue labelling hnRNP A1 with ab50492 at 4.0-8.0µg/ml. Positive staining shown in cardiac cell. Magnification: 400X.
  • Ab50492, at a concentration of 4 - 8 µg/ml, staining hnRNP A1 in paraffin embedded human liver cells by Immunohistochemistry. Hepatocytes cells are positively stained (arrows). Magnification 400x.Ab50492, at a concentration of 4 - 8 µg/ml, staining hnRNP A1 in paraffin embedded human liver cells by Immunohistochemistry. Hepatocytes cells are positively stained (arrows). Magnification 400x.
  • Ab50492, at a concentration of 4 - 8 µg/ml, staining hnRNP A1 in paraffin embedded human heart tissue by Immunohistochemistry. Myocardial cells are positively stained (arrows). Magnification 400x.Ab50492, at a concentration of 4 - 8 µg/ml, staining hnRNP A1 in paraffin embedded human heart tissue by Immunohistochemistry. Myocardial cells are positively stained (arrows). Magnification 400x.
  • Lane 1 : Marker
    Lane 2 : Anti-hnRNP A1 antibody (ab50492) at 1.25 µg/ml

    Lane 1 : As above
    Lane 2 : Jurkat cell lysate

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 39 kDa
    Observed band size : 36 kDa (why is the actual band size different from the predicted?)
    Gel concentration: 12%
  • ICC/IF image of ab50492 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab50492, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-hnRNP A1 antibody (ab50492)

This product has been referenced in:
  • Roda D  et al. EGF-Induced Acetylation of Heterogeneous Nuclear Ribonucleoproteins Is Dependent on KRAS Mutational Status in Colorectal Cancer Cells. PLoS One 10:e0130543 (2015). Human . Read more (PubMed: 26110767) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"