The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1.25 µg/ml. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Use a concentration of 4 - 8 µg/ml.
FunctionInvolved in the packaging of pre-mRNA into hnRNP particles, transport of poly(A) mRNA from the nucleus to the cytoplasm and may modulate splice site selection. May play a role in HCV RNA replication.
Post-translational modificationsArg-194, Arg-206 and Arg-225 are dimethylated, probably to asymmetric dimethylarginine. Sumoylated.
Cellular localizationNucleus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Shuttles continuously between the nucleus and the cytoplasm along with mRNA. Component of ribonucleosomes. In the course of viral infection, colocalizes with HCV NS5B at speckles in the cytoplasm in a HCV-replication dependent manner.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung tissue labelling hnRNP A1 with ab66893 at 4.0-8.0µg/ml. Positive staining shown in alveolar cells. Magnification: 400X.
Western blot - hnRNP A1 antibody (ab66893)
Anti-hnRNP A1 antibody (ab66893) at 1.25 µg/ml + Jurkat cell lysate at 10 µg
Secondary HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 34 kDa Observed band size : 34 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human intestine tissue labelling hnRNP A1 with ab66893 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of the human intestinal villus. Magnification: 400X.
References for Anti-hnRNP A1 antibody (ab66893)
has not yet been referenced specifically in any publications.
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