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Rabbit polyclonal to hnRNP A2B1
Recombinant fragment, corresponding to a region within amino acids 54-238 of Human hnRNP A2B1.
A549, HepG2, HCT116 and HeLa whole cell lysates; Paraformaldehyde fixed HeLa cells; Colon carcinoma tissue.
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.01% Thimerosal (merthiolate) Constituents: 0.75% Glycine, 1.21% Tris, 20% Glycerol
Concentration information loading...
Immunogen affinity purified
ab126197 is purified by antigen-affinity chromatography.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/10000. Predicted molecular weight: 37 kDa.
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Use 10mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0).
1/100 - 1/1000.
Involved with pre-mRNA processing. Forms complexes (ribonucleosomes) with at least 20 other different hnRNP and heterogeneous nuclear RNA in the nucleous.
Contains 2 RRM (RNA recognition motif) domains.
Nucleus > nucleoplasm. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Component of ribonucleosomes. Predominantly nucleoplasmic, however isoform A2 is also found in the cytoplasm of cells in some tissues. Not found in the nucleolus.
Information by UniProt
Heterogeneous nuclear ribonucleoprotein A2 antibody
Heterogeneous nuclear ribonucleoprotein A2/B1 antibody
Heterogeneous nuclear ribonucleoprotein B1 antibody
Western blot - Anti-hnRNP A2B1 antibody (ab126197)
Anti-hnRNP A2B1 antibody (ab126197) at 1/5000 dilution + HeLa whole cell lysate at 30 µg
Predicted band size: 37 kDa 12% SDS PAGE
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP A2B1 antibody (ab126197)
ab126197, at 1/250, staining hnRNP A2B1 in paraffin embedded Human colon carcinoma tissue by Immunohistochemistry.
Immunocytochemistry/ Immunofluorescence - Anti-hnRNP A2B1 antibody (ab126197)
ab126197, at 1/500, staining hnRNP A2B1 in paraformaldehyde fixed HeLa cells by Immunofluorescence (green). Alpha-tubulin filaments were stained red.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"