Overview

  • Product name
  • Description
    Rabbit polyclonal to hnRNP A2B1
  • Tested applications
    Suitable for: IP, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human hnRNP A2B1 aa 136-341. The exact sequence is proprietary.
    Sequence:

    GKIDT IEIITDRQSG KKRGFGFVTF DDHDPVDKIV LQKYHTINGH NAEVRKALSR QEMQEVQSSR SGRGGNFGFG DSRGGGGNFG PGPGSNFRGG SDGYGSGRGF GDGYNGYGGG PGGGNFGGSP GYGGGRGGYG GGGPGYGNQG GGYGGGYDNY GGGNYGSGNY NDFGNYNQQP SNYGPMKSGN FGGSRNMGGP YGGGNYGPGG S


    Database link: P22626

  • Positive control
    • A549, 293T , A431 , HeLa , HepG2 , A375 , Neuro2A , GL261 , C8030 , NIH-3T3 , BCL-1 , Raw264.7 , C2C12 and PC-12 whole cell lysates. U373 xenograft.

Properties

Applications

Our Abpromise guarantee covers the use of ab183654 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/100 - 1/500.
IHC-P 1/100 - 1/1000.
WB 1/5000 - 1/20000. Predicted molecular weight: 37 kDa.

Target

  • Function
    Involved with pre-mRNA processing. Forms complexes (ribonucleosomes) with at least 20 other different hnRNP and heterogeneous nuclear RNA in the nucleous.
  • Sequence similarities
    Contains 2 RRM (RNA recognition motif) domains.
  • Cellular localization
    Nucleus > nucleoplasm. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Component of ribonucleosomes. Predominantly nucleoplasmic, however isoform A2 is also found in the cytoplasm of cells in some tissues. Not found in the nucleolus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Heterogeneous nuclear ribonucleoprotein A2 antibody
    • Heterogeneous nuclear ribonucleoprotein A2/B1 antibody
    • Heterogeneous nuclear ribonucleoprotein B1 antibody
    • Heterogeneous nuclear ribonucleoproteins A2/B1 antibody
    • hnRNP A2 / hnRNP B1 antibody
    • hnRNP A2 antibody
    • hnRNP A2/B1 antibody
    • hnRNP B1 antibody
    • hnRNP-A2 antibody
    • hnRNP-B1 antibody
    • hnRNPA2 antibody
    • Hnrnpa2b1 antibody
    • hnRNPB1 antibody
    • HNRPA2 antibody
    • HNRPA2B1 antibody
    • HNRPB1 antibody
    • Nuclear ribonucleoprotein particle A2 protein antibody
    • RNP A2 antibody
    • RNP B1 antibody
    • RNP-A2 antibody
    • RNP-B1 antibody
    • RNPA2 antibody
    • RNPB1 antibody
    • ROA2_HUMAN antibody
    • SNRPB1 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded U373 xenograft labeling hnRNP A2B1 with ab183654 at 1/500 dilution.

  • All lanes : Anti-hnRNP A2B1 antibody (ab183654) at 1/10000 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : A431 whole cell lysate
    Lane 3 : HeLa whole cell lysate
    Lane 4 : HepG2 whole cell lysate
    Lane 5 : A375 whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 37 kDa
  • Anti-hnRNP A2B1 antibody (ab183654) at 1/10000 dilution + PC-12 whole cell lysate at 30 µg

    Predicted band size : 37 kDa

    12 % SDS-PAGE

  • All lanes : Anti-hnRNP A2B1 antibody (ab183654) at 1/10000 dilution

    Lane 1 : Neuro2A whole cell lysate
    Lane 2 : GL261 whole cell lysate
    Lane 3 : C8D30 whole cell lysate
    Lane 4 : NIH-3T3 whole cell lysate
    Lane 5 : BCL-1 whole cell lysate
    Lane 6 : Raw264.7 cell lysate
    Lane 7 : C2C12 whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 37 kDa

    12 % SDS-PAGE

  • Western blot analysis using ab183654 at 1/200 dilution detecting hnRNP A2B1 in immunoprecipitation pellets from 4500 µg A549 whole cell lysate with ab183654 at 2 µg. Lane 2 shows Control with 2 μg of preimmune rabbit IgG.

References

ab183654 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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