Anti-hnRNP A2B1 antibody [DP3B3] (ab6102)

  Western blot - hnRNP A2B1 antibody [DP3B3] (ab6102)

All lanes : Anti-hnRNP A2B1 antibody [DP3B3] (ab6102) at 1/1000 dilution

Lane 1 : HeLa (hnRNPA2 shRNA-1) at 1/1000 dilution
Lane 2 : HeLa (hnRNPA2 shRNA-1)
Lane 3 : HeLa hnRNPA2 shRNA-2 at 1/1000 dilution
Lane 4 : HeLa hnRNPA2 shRNA-2
Lane 5 : HeLa (Control shRNA)
Lane 6 : HeLa (Control shRNA)
Lane 7 : Untreated HeLa

Secondary
HRP-conjugated Goat anti-mouse polyclonal at 1/1000 dilution

Predicted band size : 37 kDa

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  Immunocytochemistry/ Immunofluorescence - hnRNP A2B1 antibody [DP3B3] (ab6102)

ab6102 staining hnRNP A2B1 in human A549 cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed with formaldehyde, permeabilized with Triton X-100 and blocking with 10% serum at 20⁰C for 1 hour was performed. Samples were incubated with primary antibody (1/100: in PBS containing 10% FCS) for 1 hour at 20⁰C. An Alexa Fluor ® 488-conjugated goat polyclonal to mouse IgG was used at dilution at 1/300 as secondary antibody.

This image is a courtesy of Anonymous Abreview

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - hnRNP A2B1 antibody [DP3B3] (ab6102)

ab6102 (1µg/ml) staining hnRNP A2B1 in human liver.
Sections were stained using an automated system (DAKO Autostainer Plus), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.1 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - hnRNP A2B1 antibody [DP3B3] (ab6102)

ab6102 (1µg/ml) staining hnRNP A2B1 in human caecum, using an automated system (DAKO Autostainer Plus). Using this protocol there is strong nuclear staining in the mucosal epithelium.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.