Overview

  • Product nameAnti-hnRNP A2B1 antibody [DP3B3]
    See all hnRNP A2B1 primary antibodies
  • Description
    Mouse monoclonal [DP3B3] to hnRNP A2B1
  • Tested applicationsSuitable for: ICC/IF, IHC-Fr, ELISA, WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Chicken, Human, Pig
  • Immunogen

    Bacterially expressed His-hnRNPA2 fusion protein (Human).

Properties

Applications

Our Abpromise guarantee covers the use of ab6102 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
IHC-Fr 1/100. PubMed: 17067748Fix with acetone.
ELISA Use at an assay dependent concentration.
WB 1/1500 - 1/6000. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa).
IP Use at an assay dependent concentration.
IHC-P Use a concentration of 1 µg/ml.

Target

  • FunctionInvolved with pre-mRNA processing. Forms complexes (ribonucleosomes) with at least 20 other different hnRNP and heterogeneous nuclear RNA in the nucleous.
  • Sequence similaritiesContains 2 RRM (RNA recognition motif) domains.
  • Cellular localizationNucleus > nucleoplasm. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Component of ribonucleosomes. Predominantly nucleoplasmic, however isoform A2 is also found in the cytoplasm of cells in some tissues. Not found in the nucleolus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Heterogeneous nuclear ribonucleoprotein A2 antibody
    • Heterogeneous nuclear ribonucleoprotein A2/B1 antibody
    • Heterogeneous nuclear ribonucleoprotein B1 antibody
    • Heterogeneous nuclear ribonucleoproteins A2/B1 antibody
    • hnRNP A2 / hnRNP B1 antibody
    • hnRNP A2 antibody
    • hnRNP A2/B1 antibody
    • hnRNP B1 antibody
    • hnRNP-A2 antibody
    • hnRNP-B1 antibody
    • hnRNPA2 antibody
    • Hnrnpa2b1 antibody
    • hnRNPB1 antibody
    • HNRPA2 antibody
    • HNRPA2B1 antibody
    • HNRPB1 antibody
    • Nuclear ribonucleoprotein particle A2 protein antibody
    • RNP A2 antibody
    • RNP B1 antibody
    • RNP-A2 antibody
    • RNP-B1 antibody
    • RNPA2 antibody
    • RNPB1 antibody
    • ROA2_HUMAN antibody
    • SNRPB1 antibody
    see all

Anti-hnRNP A2B1 antibody [DP3B3] images

  • All lanes : Anti-hnRNP A2B1 antibody [DP3B3] (ab6102) at 1/1000 dilution

    Lane 1 : HeLa (hnRNPA2 shRNA-1) at 1/1000 dilution
    Lane 2 : HeLa (hnRNPA2 shRNA-1)
    Lane 3 : HeLa hnRNPA2 shRNA-2 at 1/1000 dilution
    Lane 4 : HeLa hnRNPA2 shRNA-2
    Lane 5 : HeLa (Control shRNA)
    Lane 6 : HeLa (Control shRNA)
    Lane 7 : Untreated HeLa

    Secondary
    HRP-conjugated Goat anti-mouse polyclonal at 1/1000 dilution

    Predicted band size : 37 kDa

    This image is courtesy of an anonymous Abreview

    See Abreview

  • ab6102 staining hnRNP A2B1 in human A549 cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed with formaldehyde, permeabilized with Triton X-100 and blocking with 10% serum at 200C for 1 hour was performed. Samples were incubated with primary antibody (1/100: in PBS containing 10% FCS) for 1 hour at 200C. An Alexa Fluor ® 488-conjugated goat polyclonal to mouse IgG was used at dilution at 1/300 as secondary antibody.

    See Abreview

  • ab6102 (1µg/ml) staining hnRNP A2B1 in human liver.
    Sections were stained using an automated system (DAKO Autostainer Plus), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.1 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
  • ab6102 (1µg/ml) staining hnRNP A2B1 in human caecum, using an automated system (DAKO Autostainer Plus). Using this protocol there is strong nuclear staining in the mucosal epithelium.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

References for Anti-hnRNP A2B1 antibody [DP3B3] (ab6102)

This product has been referenced in:
  • Cortese A  et al. Widespread RNA metabolism impairment in sporadic inclusion body myositis TDP43-proteinopathy. Neurobiol Aging 35:1491-8 (2014). IHC-Fr ; Human . Read more (PubMed: 24462217) »
  • Singh NN  et al. An intronic structure enabled by a long-distance interaction serves as a novel target for splicing correction in spinal muscular atrophy. Nucleic Acids Res 41:8144-65 (2013). WB . Read more (PubMed: 23861442) »

See all 13 Publications for this product

Product Wall

Thank you for your enquiry regarding ab6102 and for taking the time to provide some useful details of the experiments. I am very sorry to hear that you are having problems with this antibody. Band at 37 kDa in input sample indicate that the antibod...

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Application Immunocytochemistry
Sample Mouse Cell (Retina)
Permeabilization Yes - freeze/thaw
Specification Retina
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative Paraformaldehyde
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Submitted Oct 27 2015

AB18293; Rabbit anti- NRA52/LRH-1 Lot GR9515-6 = 0.290 mg/ml;  Lot: GR34910-1 = 0.220 mg/ml AB10685; Mouse anti- hnRNPA1 [4B10] Lot: GR14303-5 =  1 mg/ml AB6102; Mouse anti- hnRNPA2/B1 [DP3B3] Lot: GR21753-3 = 1 mg/ml AB16667 Rabbit anti- Ki67...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused...

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For dispatching the requested product, we would need the order number of original purchase. Could you please send this asap? Looking forward to hearing from you soon.

Thank you very much for sending the image and all the details. I acknowledge the time you have spent trying this antibody and convinced that the observed band size is not at 37 kDa. The antibody should be detecting the band at right size; the rat p...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Human Bladder Cancer)
Specification Human Bladder Cancer
Fixative Formaldehyde
Antigen retrieval step Heat mediated
Permeabilization No
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Submitted May 11 2011

Application Western blot
Sample Mouse Cell lysate - whole cell (NSC34)
Loading amount 10 µg
Specification NSC34
Gel Running Conditions Reduced Denaturing (4-12% nupage gel)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Nov 12 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa)
Loading amount 30 µg
Specification HeLa
Gel Running Conditions Reduced Denaturing (12.5%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Nov 26 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (A549)
Specification A549
Fixative Formaldehyde
Permeabilization Yes - tx 100
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
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Submitted Jun 05 2009

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