Overview

  • Product name
    Anti-hnRNP C1 + C2 antibody [4F4]
    See all hnRNP C1 + C2 primary antibodies
  • Description
    Mouse monoclonal [4F4] to hnRNP C1 + C2
  • Specificity
    This antibody recognizes the two hnRNP C proteins of 41kDa (C1) and 43kDa (C2) in human cells, and corresponding proteins in monkey hamster & chicken.
  • Tested applications
    Suitable for: ICC/IF, IP, IHC-P, ELISA, WBmore details
  • Species reactivity
    Reacts with: Mouse, Chicken, Hamster, Human, Monkey
  • Immunogen

    RNP’s eluted from oligo (dT) cellulose column

Properties

Applications

Our Abpromise guarantee covers the use of ab10294 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ELISA Use at an assay dependent concentration.
WB 1/1000. Detects a band of approximately 36 kDa (predicted molecular weight: 41,43 kDa). Human nuclear HEK 293A lysate (see Abreview). Detects isoforms at molecular weight approximately 41,43 kDa.

Target

  • Function
    Binds pre-mRNA and nucleates the assembly of 40S hnRNP particles (PubMed:8264621). Specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs, a modification present at internal sites of mRNAs that affects mRNA splicing, processing and stability. M6A alters the local structure in mRNAs and long non-coding RNAs (lncRNAs) via a mechanism named 'm(6)A-switch' to facilitate binding of HNRNPC, leading to regulation of mRNA splicing (PubMed:25719671). Single HNRNPC tetramers bind 230-240 nucleotides. Trimers of HNRNPC tetramers bind 700 nucleotides. May play a role in the early steps of spliceosome assembly and pre-mRNA splicing. Interacts with poly-U tracts in the 3'-UTR or 5'-UTR of mRNA and modulates the stability and the level of translation of bound mRNA molecules (PubMed:12509468, PubMed:16010978, PubMed:7567451, PubMed:8264621).
  • Sequence similarities
    Belongs to the RRM HNRPC family. RALY subfamily.
    Contains 1 RRM (RNA recognition motif) domain.
  • Post-translational
    modifications
    Phosphorylated on Ser-260 and Ser-299 in resting cells. Phosphorylated on Ser-253 and on 1 serine residue in the poly-Ser stretch at position 238 in response to hydrogen peroxide.
    Sumoylated. Sumoylation reduces affinity for mRNA.
  • Cellular localization
    Nucleus. Component of ribonucleosomes.
  • Information by UniProt
  • Database links
  • Alternative names
    • C1 antibody
    • C2 antibody
    • Heterogeneous nuclear ribonucleoprotein C (C1/C2) antibody
    • Heterogeneous nuclear ribonucleoprotein C antibody
    • Heterogeneous nuclear ribonucleoproteins C1/C2 antibody
    • HNRNP antibody
    • hnRNP C1 / hnRNP C2 antibody
    • hnRNP C1/C2 antibody
    • Hnrnpc antibody
    • HNRPC antibody
    • HNRPC_HUMAN antibody
    • MGC104306 antibody
    • MGC105117 antibody
    • MGC117353 antibody
    • MGC131677 antibody
    • Nuclear ribonucleoprotein particle C1 protein antibody
    • Nuclear ribonucleoprotein particle C2 protein antibody
    • SNRPC antibody
    see all

Anti-hnRNP C1 + C2 antibody [4F4] images

  • All lanes : Anti-hnRNP C1 + C2 antibody [4F4] (ab10294) at 1/1000 dilution

    Lane 1 : Nuclear lysate, HEK293A cells
    Lane 2 : Cytoplasmic lysate, HEK293A cells

    Lysates/proteins at 30 µg per lane.

    Secondary
    HRP conjugated ImmunoPure Goat Anti-Mouse IgG (H+L) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 41,43 kDa
    Observed band size : 42,43 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 second

    This image is courtesy of an anonymous Abreview

    See Abreview

  • ab10294 (1µg/ml) staining hnRNP in human placenta, using an automated system (DAKO Autostainer Plus). Using this protocol there is strong nuclear staining.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH6.1 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

References for Anti-hnRNP C1 + C2 antibody [4F4] (ab10294)

This product has been referenced in:

See all 21 Publications for this product

Product Wall

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - other (B Cells)
Specification
B Cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Ms. Jennifer Blancas

Verified customer

Submitted Sep 10 2014

Application
Western blot
Loading amount
15 µg
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (4-12% Bis-Tris Gel, MES running buffer)
Sample
Human Cell lysate - whole cell (Human Embryonic Kidney)
Specification
Human Embryonic Kidney
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Mar 18 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - nuclear (U87 (Glioblastoma))
Loading amount
100 µg
Specification
U87 (Glioblastoma)
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Nov 16 2011

Application
Western blot
Sample
Mouse Cell lysate - nuclear (NSC34)
Loading amount
10 µg
Specification
NSC34
Gel Running Conditions
Reduced Denaturing (4-12% nupage gel)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Nov 24 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK 293 cells)
Loading amount
20 µg
Specification
HEK 293 cells
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Username

Dr. Sarah Ronnebaum

Verified customer

Submitted May 22 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Renal carcinoma cell line)
Specification
Renal carcinoma cell line
Fixative
Paraformaldehyde
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 4%
Username

Abcam user community

Verified customer

Submitted Oct 18 2006

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - nuclear (HEK 293A)
Loading amount
35 µg
Specification
HEK 293A
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4%
Username

Abcam user community

Verified customer

Submitted Jul 21 2006

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Hamster Cell lysate - nuclear (CHO (Chinese Hamster Ovary) cell line)
Loading amount
35 µg
Specification
CHO (Chinese Hamster Ovary) cell line
Treatment
Fractionation by Dounce homogenization
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4%
Username

Abcam user community

Verified customer

Submitted Jul 10 2006

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (U2OS cell line (osteosarcoma))
Specification
U2OS cell line (osteosarcoma)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4%
Username

Abcam user community

Verified customer

Submitted Dec 05 2005

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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