Overview

  • Product nameAnti-hnRNP C1 + C2 antibody [EP3034Y]
    See all hnRNP C1 + C2 primary antibodies
  • Description
    Rabbit monoclonal [EP3034Y] to hnRNP C1 + C2
  • Tested applicationsSuitable for: WB, IHC-P, ICCmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues near the N-terminus of human hnRNP C1 + C2

  • Positive control
    • HeLa, 293T, MCF7 and human cerebellum lysates; human breast carcinoma tissue.
  • General notes

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Applications

Our Abpromise guarantee covers the use of ab75822 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50000 - 1/100000. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC 1/100 - 1/250.
  • Application notesIs unsuitable for Flow Cyt or IP.
  • Target

    • FunctionBinds pre-mRNA and nucleates the assembly of 40S hnRNP particles (PubMed:8264621). Specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs, a modification present at internal sites of mRNAs that affects mRNA splicing, processing and stability. M6A alters the local structure in mRNAs and long non-coding RNAs (lncRNAs) via a mechanism named 'm(6)A-switch' to facilitate binding of HNRNPC, leading to regulation of mRNA splicing (PubMed:25719671). Single HNRNPC tetramers bind 230-240 nucleotides. Trimers of HNRNPC tetramers bind 700 nucleotides. May play a role in the early steps of spliceosome assembly and pre-mRNA splicing. Interacts with poly-U tracts in the 3'-UTR or 5'-UTR of mRNA and modulates the stability and the level of translation of bound mRNA molecules (PubMed:12509468, PubMed:16010978, PubMed:7567451, PubMed:8264621).
    • Sequence similaritiesBelongs to the RRM HNRPC family. RALY subfamily.
      Contains 1 RRM (RNA recognition motif) domain.
    • Post-translational
      modifications
      Phosphorylated on Ser-260 and Ser-299 in resting cells. Phosphorylated on Ser-253 and on 1 serine residue in the poly-Ser stretch at position 238 in response to hydrogen peroxide.
      Sumoylated. Sumoylation reduces affinity for mRNA.
    • Cellular localizationNucleus. Component of ribonucleosomes.
    • Information by UniProt
    • Database links
    • Alternative names
      • C1 antibody
      • C2 antibody
      • Heterogeneous nuclear ribonucleoprotein C (C1/C2) antibody
      • Heterogeneous nuclear ribonucleoprotein C antibody
      • Heterogeneous nuclear ribonucleoproteins C1/C2 antibody
      • HNRNP antibody
      • hnRNP C1 / hnRNP C2 antibody
      • hnRNP C1/C2 antibody
      • Hnrnpc antibody
      • HNRPC antibody
      • HNRPC_HUMAN antibody
      • MGC104306 antibody
      • MGC105117 antibody
      • MGC117353 antibody
      • MGC131677 antibody
      • Nuclear ribonucleoprotein particle C1 protein antibody
      • Nuclear ribonucleoprotein particle C2 protein antibody
      • SNRPC antibody
      see all

    Anti-hnRNP C1 + C2 antibody [EP3034Y] images

    • ab75822 at 1/100 dilution staining hnRNP C1 + C2 in human breast carcinoma by Immunohistochemistry, Paraffin-embedded tissue.
    • All lanes : Anti-hnRNP C1 + C2 antibody [EP3034Y] (ab75822) at 1/100000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : MCF7 cell lysate
      Lane 4 : human cerebellum lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 34 kDa
      Observed band size : 34 kDa

    References for Anti-hnRNP C1 + C2 antibody [EP3034Y] (ab75822)

    This product has been referenced in:
    • Akhrymuk I  et al. Evasion of the innate immune response: the Old World alphavirus nsP2 protein induces rapid degradation of Rpb1, a catalytic subunit of RNA polymerase II. J Virol 86:7180-91 (2012). WB . Read more (PubMed: 22514352) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"