The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50000 - 1/100000. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
1/100 - 1/250.
Application notesIs unsuitable for Flow Cyt or IP.
FunctionBinds pre-mRNA and nucleates the assembly of 40S hnRNP particles (PubMed:8264621). Specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs, a modification present at internal sites of mRNAs that affects mRNA splicing, processing and stability. M6A alters the local structure in mRNAs and long non-coding RNAs (lncRNAs) via a mechanism named 'm(6)A-switch' to facilitate binding of HNRNPC, leading to regulation of mRNA splicing (PubMed:25719671). Single HNRNPC tetramers bind 230-240 nucleotides. Trimers of HNRNPC tetramers bind 700 nucleotides. May play a role in the early steps of spliceosome assembly and pre-mRNA splicing. Interacts with poly-U tracts in the 3'-UTR or 5'-UTR of mRNA and modulates the stability and the level of translation of bound mRNA molecules (PubMed:12509468, PubMed:16010978, PubMed:7567451, PubMed:8264621).
Sequence similaritiesBelongs to the RRM HNRPC family. RALY subfamily. Contains 1 RRM (RNA recognition motif) domain.
Post-translational modificationsPhosphorylated on Ser-260 and Ser-299 in resting cells. Phosphorylated on Ser-253 and on 1 serine residue in the poly-Ser stretch at position 238 in response to hydrogen peroxide. Sumoylated. Sumoylation reduces affinity for mRNA.
Cellular localizationNucleus. Component of ribonucleosomes.
ab75822 at 1/100 dilution staining hnRNP C1 + C2 in human
breast carcinoma by Immunohistochemistry, Paraffin-embedded tissue.
Western blot - hnRNP C1 + C2 antibody [EP3034Y] (ab75822)
All lanes : Anti-hnRNP C1 + C2 antibody [EP3034Y] (ab75822) at 1/100000 dilution
Lane 1 : HeLa cell lysate Lane 2 : 293T cell lysate Lane 3 : MCF7 cell lysate Lane 4 : human cerebellum lysate
Lysates/proteins at 10 µg per lane.
Secondary goat anti-rabbit HRP at 1/2000 dilution
Predicted band size : 34 kDa Observed band size : 34 kDa
References for Anti-hnRNP C1 + C2 antibody [EP3034Y] (ab75822)
This product has been referenced in:
Akhrymuk I et al. Evasion of the innate immune response: the Old World alphavirus nsP2 protein induces rapid degradation of Rpb1, a catalytic subunit of RNA polymerase II. J Virol86:7180-91 (2012).
Read more (PubMed: 22514352) »