Overview

  • Product nameAnti-hnRNP C1 + C2 (phospho S260) antibody
    See all hnRNP C1 + C2 primary antibodies
  • Description
    Rabbit polyclonal to hnRNP C1 + C2 (phospho S260)
  • Specificityab61796 detects endogenous levels of hnRNP C1 + C2 only when phosphorylated at serine 260.
  • Tested applicationsSuitable for: ELISA, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic phosphopeptide derived from human hnRNP C1 + C2 around the phosphorylation site of serine 260 (D-D-SP-A-E).

  • Positive control
    • Human brain tissue and 293 cell extracts treated with H2O2 (100uM, 15mins).

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab61796 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab61796 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
IHC-P
WB
  • Application notesELISA: 1/20000.
    IHC-P: 1/50 - 1/100.
    WB: 1/500 - 1/1000. Detects bands of approximately 40, 45 kDa (predicted molecular weight: 34 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionBinds pre-mRNA and nucleates the assembly of 40S hnRNP particles (PubMed:8264621). Specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs, a modification present at internal sites of mRNAs that affects mRNA splicing, processing and stability. M6A alters the local structure in mRNAs and long non-coding RNAs (lncRNAs) via a mechanism named 'm(6)A-switch' to facilitate binding of HNRNPC, leading to regulation of mRNA splicing (PubMed:25719671). Single HNRNPC tetramers bind 230-240 nucleotides. Trimers of HNRNPC tetramers bind 700 nucleotides. May play a role in the early steps of spliceosome assembly and pre-mRNA splicing. Interacts with poly-U tracts in the 3'-UTR or 5'-UTR of mRNA and modulates the stability and the level of translation of bound mRNA molecules (PubMed:12509468, PubMed:16010978, PubMed:7567451, PubMed:8264621).
    • Sequence similaritiesBelongs to the RRM HNRPC family. RALY subfamily.
      Contains 1 RRM (RNA recognition motif) domain.
    • Post-translational
      modifications
      Phosphorylated on Ser-260 and Ser-299 in resting cells. Phosphorylated on Ser-253 and on 1 serine residue in the poly-Ser stretch at position 238 in response to hydrogen peroxide.
      Sumoylated. Sumoylation reduces affinity for mRNA.
    • Cellular localizationNucleus. Component of ribonucleosomes.
    • Information by UniProt
    • Database links
    • Alternative names
      • C1 antibody
      • C2 antibody
      • Heterogeneous nuclear ribonucleoprotein C (C1/C2) antibody
      • Heterogeneous nuclear ribonucleoprotein C antibody
      • Heterogeneous nuclear ribonucleoproteins C1/C2 antibody
      • HNRNP antibody
      • hnRNP C1 / hnRNP C2 antibody
      • hnRNP C1/C2 antibody
      • Hnrnpc antibody
      • HNRPC antibody
      • HNRPC_HUMAN antibody
      • MGC104306 antibody
      • MGC105117 antibody
      • MGC117353 antibody
      • MGC131677 antibody
      • Nuclear ribonucleoprotein particle C1 protein antibody
      • Nuclear ribonucleoprotein particle C2 protein antibody
      • SNRPC antibody
      see all

    Anti-hnRNP C1 + C2 (phospho S260) antibody images

    • All lanes : Anti-hnRNP C1 + C2 (phospho S260) antibody (ab61796) at 1/500 dilution

      Lane 1 : 293 cell extracts treated with H2O2 (100uM, 15mins)
      Lane 2 : 293 cell extracts treated with H2O2 (100uM, 15mins) with immunising peptide at 5 µg

      Lysates/proteins at 5 µg per lane.


      Predicted band size : 34 kDa
      Observed band size : 45 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 40 kDa. We are unsure as to the identity of these extra bands.
    • ab61796 at 1/50 - 1/100 dilution staining hnRNP C1 + C2 in human brain Left apical lobe by Immunohistochemistry, Paraffin-embedded tissue, in the absence or presence of the immunising peptide.

    References for Anti-hnRNP C1 + C2 (phospho S260) antibody (ab61796)

    This product has been referenced in:
    • Chen S  et al. Reduced expression of lamin A/C results in modified cell signaling and metabolism coupled with changes in expression of structural proteins. J Proteome Res 8:5196-211 (2009). Read more (PubMed: 19775189) »

    See 1 Publication for this product

    Product Wall

    There are currently no Abreviews or Questions for ab61796.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"