Recombinant full length Human hnRNP F produced in HEK293T cells (NP_004957).
HEK293T cell lysate transfected with pCMV6-ENTRY hnRNP F cDNA; Human Kidney, liver, ovary adenocarcinoma, prostate, and bladder carcinoma tissues; COS7 cells transiently transfected by pCMV6-ENTRY hnRNP F;
HepG2, HeLa and Jurkat cells.
General notesDilute in PBS (pH7.3) before use. Stable for 12 months from date of receipt.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000. Predicted molecular weight: 46 kDa.
1/100. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
1/50 - 1/100.
FunctionComponent of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. Plays a role in the regulation of alternative splicing events. Binds G-rich sequences in pre-mRNAs and keeps target RNA in an unfolded state.