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Our Abpromise guarantee covers the use of ab10689 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ELISA||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Detects a band of approximately 53 kDa (hnRNP F), 56 kDa (hnRNP H). (predicted molecular weight: hnRNP F = 49.8 kDa, hnRNP H = 53.9kDa).|
|IP||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.|