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Immunogen was a synthetic peptide, which represented a portion of the C-terminus of human heterogeneous nuclear ribonucleoprotein H1 (LocusLink ID 3187).
Our Abpromise guarantee covers the use of ab10374 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/30000. Detects a band of approximately 49 kDa (predicted molecular weight: 53 kDa).|
|IP||Use at 4-8 µg/mg of lysate.|
|IHC-Fr||Use at an assay dependent concentration. PubMed: 24462217|
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||1/500. PubMed: 20020773|
ab10374 detection of human hnRNPH by Western Blot. Samples: Nuclear extract (NE; 40