Overview

  • Product name
  • Description
    Rabbit polyclonal to hnRNP H
  • Specificity
    The antibody was designed against hnRNPH1 and does not recognize hnRNPH3. The epitope recognized by ab10374 maps to a region between residue 400 and the C-terminus (residue 448) of human Heterogeneous Nuclear Ribonucleoprotein H1 using the numbering given in entry NP_005511.1 (GeneID 3187).
  • Tested applications
    Suitable for: WB, IP, IHC-Fr, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Opossum, Orangutan, Elephant
  • Immunogen

    Immunogen was a synthetic peptide, which represented a portion of the C-terminus of human heterogeneous nuclear ribonucleoprotein H1 (LocusLink ID 3187).

  • Positive control
    • nuclear lysate from HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab10374 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/30000. Detects a band of approximately 49 kDa (predicted molecular weight: 53 kDa).
IP Use at 4-8 µg/mg of lysate.
IHC-Fr Use at an assay dependent concentration. PubMed: 24462217
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/500. PubMed: 20020773

Target

  • Function
    This protein is a component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. Mediates pre-mRNA alternative splicing regulation. Inhibits, together with CUGBP1, insulin receptor (IR) pre-mRNA exon 11 inclusion in myoblast. Binds to the IR RNA. Binds poly(RG).
  • Tissue specificity
    Expressed ubiquitously.
  • Sequence similarities
    Contains 3 RRM (RNA recognition motif) domains.
  • Domain
    Each quasi-RRM repeat bound poly(RG), while only the N-terminal QRRM bound poly(RC) and poly(RU). None of the repeats bound detectable amounts of poly(RA).
  • Cellular localization
    Nucleus > nucleoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp686A15170 antibody
    • Heterogeneous nuclear ribonucleoprotein H antibody
    • Heterogeneous nuclear ribonucleoprotein H1 (H) antibody
    • Heterogeneous nuclear ribonucleoprotein H1 antibody
    • HNRH1_HUMAN antibody
    • hnRNP H antibody
    • hnRNPH antibody
    • Hnrnph1 antibody
    • HNRPH 1 antibody
    • HNRPH antibody
    • HNRPH1 protein antibody
    • N-terminally processed antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling hnRNP H with ab10374 at 1/1000 (1µg/ml). Detection: DAB.


  • Predicted band size : 53 kDa

    ab10374 detection of human hnRNPH by Western Blot. Samples: Nuclear extract (NE; 40 µg) from HeLa cells and recombinant human hnRNPH (rH; ~100 ng). Antibody: ab10374 used at the indicated concentrations. Detection: Protein A – HRP with chemiluminescence (20 second exposure after a 20 minute delay to allow signal to decay).

    ab10374 detection of human hnRNPH by Western Blot. Samples: Nuclear extract (NE; 40 µg) from HeLa cells and recombinant human hnRNPH (rH; ~100 ng). Antibody: ab10374 used at the indicated concentrations. Detection: Protein A – HRP with chemiluminescence (20 second exposure after a 20 minute delay to allow signal to decay).
  • ICC/IF image of ab10374 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab10374, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • IHC image of ab10374 staining in human normal cervical carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab10374, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References

This product has been referenced in:
  • Ajith S  et al. Position-dependent activity of CELF2 in the regulation of splicing and implications for signal-responsive regulation in T cells. RNA Biol 13:569-81 (2016). Read more (PubMed: 27096301) »
  • Sun YL  et al. Protein and gene expression characteristics of heterogeneous nuclear ribonucleoprotein H1 in esophageal squamous cell carcinoma. World J Gastroenterol 22:7322-31 (2016). Read more (PubMed: 27621578) »

See all 14 Publications for this product

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (c2c12 cells)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
20 µg
Specification
c2c12 cells
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Nov 20 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (U-2OS)
Permeabilization
No
Specification
U-2OS
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
Methanol
Username

Abcam user community

Verified customer

Submitted Jun 09 2017

Thank you for contacting Abcam. We have not tested this antibody specifically with hnRNP F/A/B. However, It is unlikely that this antibody will react with the other family members. If there is anything else I can help you with, please let me know.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Human Cell lysate - other (HeLa cytosolic extract)
Total protein in input
500 µg
Specification
HeLa cytosolic extract
Immuno-precipitation step
Protein A
Username

Abcam user community

Verified customer

Submitted Oct 30 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa cytosolic and nuclear extracts)
Loading amount
50 µg
Specification
HeLa cytosolic and nuclear extracts
Gel Running Conditions
Reduced Denaturing (8% gel)
Blocking step
Milk as blocking agent for 14 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Oct 30 2009

Thank you for your enquiry. The epitope recognized by ab10374 has been mapped to a region between residue 400 and the C-terminus (residue 448) of human Heterogeneous Nuclear Ribonucleoprotein H1 using the numbering given in entry NP_005511.1 (GeneI...

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Thank you for your enquiry. The antibody ab10374 was designed to recognize hnRNPH1 and does not recognize hnRNPH3. This information has now been added on our datasheet. Please do not hesitate to contact us again if you require further information,

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I'm sorry to hear you are experiencing a problem with ab10374 in WB. You are absolutely correct, there was indeed an error on the datasheet and it has been corrected to " use at a dilution of 1:10 000 to 1:30 000, therefore this would be equivalent...

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