• Product nameAnti-hnRNP K antibody [3C2] - ChIP Grade
    See all hnRNP K primary antibodies
  • Description
    Mouse monoclonal [3C2] to hnRNP K - ChIP Grade
  • Tested applicationsSuitable for: WB, IP, ELISA, ICC/IF, Flow Cyt, ChIP/Chipmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Hamster, Cow, Human, Xenopus laevis
  • Immunogen

    Full length native protein (purified) (Human)

  • Positive control
    • HeLa extracts. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: HeLa.



Our Abpromise guarantee covers the use of ab39975 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 65 kDa (predicted molecular weight: 51 kDa).
IP Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use 1µg for 106 cells. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
ChIP/Chip Use at an assay dependent concentration. PubMed: 20673990


  • FunctionOne of the major pre-mRNA-binding proteins. Binds tenaciously to poly(C) sequences. Likely to play a role in the nuclear metabolism of hnRNAs, particularly for pre-mRNAs that contain cytidine-rich sequences. Can also bind poly(C) single-stranded DNA.
  • Sequence similaritiesContains 3 KH domains.
  • Post-translational
    Arg-296 and Arg-299 are dimethylated, probably to asymmetric dimethylarginine.
  • Cellular localizationCytoplasm. Nucleus > nucleoplasm. In case of ASFV infection, there is a shift in the localization which becomes predominantly nuclear.
  • Information by UniProt
  • Database links
  • Alternative names
    • CSBP antibody
    • dC stretch binding protein antibody
    • FLJ41122 antibody
    • Heterogeneous nuclear ribonucleoprotein K antibody
    • hnRNP K antibody
    • HNRNPK antibody
    • HNRPK antibody
    • HNRPK_HUMAN antibody
    • Transformation up regulated nuclear protein antibody
    • Transformation up-regulated nuclear protein antibody
    • Transformation upregulated nuclear protein antibody
    • TUNP antibody
    see all

Anti-hnRNP K antibody [3C2] - ChIP Grade images

  • ICC/IF image of ab39975 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab39975 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- mouse (ab96879) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-hnRNP K antibody [3C2] - ChIP Grade (ab39975) at 1/1000 dilution

    Lane 1 : SK N BE (Human neuroblastoma) Whole Cell Lysate
    Lane 2 : TE 671 (Human Rhabdomyosarcoma) Whole Cell Lysate
    Lane 3 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate

    Lysates/proteins at 10 µg per lane.

    Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size : 51 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 65 kDa (possible post-translational modification).
  • Overlay histogram showing HeLa cells stained with ab39975 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab39975, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References for Anti-hnRNP K antibody [3C2] - ChIP Grade (ab39975)

This product has been referenced in:
  • Thompson PJ  et al. hnRNP K Coordinates Transcriptional Silencing by SETDB1 in Embryonic Stem Cells. PLoS Genet 11:e1004933 (2015). WB, IP, ICC/IF . Read more (PubMed: 25611934) »
  • Fan B  et al. A human proteome microarray identifies that the heterogeneous nuclear ribonucleoprotein K (hnRNP K) recognizes the 5' terminal sequence of the hepatitis C virus RNA. Mol Cell Proteomics 13:84-92 (2014). Human . Read more (PubMed: 24113282) »

See all 5 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Mouse Cell lysate - nuclear (Embryonic stem cells)
Total protein in input 300 µg
Specification Embryonic stem cells
Immuno-precipitation step Protein G

Mr. Peter Thompson

Verified customer

Submitted May 25 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - nuclear (NSC34)
Loading amount 10 µg
Specification NSC34
Gel Running Conditions Reduced Denaturing (4-12% nupage gel)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Dec 02 2010