For the best experience on the Abcam website please upgrade to a modern browser such as
Rabbit polyclonal to hnRNP K
Predicted to work with:
Mouse, Rat, Rabbit, Cow, Dog, Pig, Rhesus monkey
Recombinant fragment within Human hnRNP K (internal sequence). The exact sequence is proprietary.
Database link: P61978
WB: HEK-293T whole cell lysate.
ICC/IF: HeLa cells.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol, 0.75% Glycine, 1.21% Tris
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/3000. Predicted molecular weight: 50 kDa.
1/100 - 1/1000.
One of the major pre-mRNA-binding proteins. Binds tenaciously to poly(C) sequences. Likely to play a role in the nuclear metabolism of hnRNAs, particularly for pre-mRNAs that contain cytidine-rich sequences. Can also bind poly(C) single-stranded DNA.
Contains 3 KH domains.
Arg-296 and Arg-299 are dimethylated, probably to asymmetric dimethylarginine.
Cytoplasm. Nucleus > nucleoplasm. In case of ASFV infection, there is a shift in the localization which becomes predominantly nuclear.
Information by UniProt
dC stretch binding protein antibody
Western blot - Anti-hnRNP K antibody (ab227103)
Anti-hnRNP K antibody (ab227103) at 1/1000 dilution + HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg
Predicted band size:
10% SDS-PAGE gel.
Immunocytochemistry/ Immunofluorescence - Anti-hnRNP K antibody (ab227103)
Paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling hnRNP K (green) using ab227103 in ICC/IF.
Nuclear counterstain: Hoechst 33342.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"