The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
1/2000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 51 kDa).
Use at 1-4 µg/mg of lysate.
FunctionOne of the major pre-mRNA-binding proteins. Binds tenaciously to poly(C) sequences. Likely to play a role in the nuclear metabolism of hnRNAs, particularly for pre-mRNAs that contain cytidine-rich sequences. Can also bind poly(C) single-stranded DNA.
Sequence similaritiesContains 3 KH domains.
Post-translational modificationsArg-296 and Arg-299 are dimethylated, probably to asymmetric dimethylarginine.
Cellular localizationCytoplasm. Nucleus > nucleoplasm. In case of ASFV infection, there is a shift in the localization which becomes predominantly nuclear.
Heterogeneous nuclear ribonucleoprotein K antibody
hnRNP K antibody
Transformation up regulated nuclear protein antibody
Transformation up-regulated nuclear protein antibody
Transformation upregulated nuclear protein antibody
Anti-hnRNP K antibody images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP K antibody (ab70490)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma (left) and mouse squamous cell carcinoma (right) tissue labelling hnRNP K with ab70490 at 1/200 (1µg/ml) and 1/1000 (0.2µg/ml). Detection: DAB.
Western blot - hnRNP K antibody (ab70490)
All lanes : Anti-hnRNP K antibody (ab70490) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : 293T whole cell lysate at 50 µg Lane 5 : NIH3T3 whole cell lysate at 50 µg