Anti-hnRNP K antibody (ab70492)
Key features and details
- Rabbit polyclonal to hnRNP K
- Suitable for: WB, IP, ICC/IF, IHC-P, ChIP/Chip
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-hnRNP K antibody
See all hnRNP K primary antibodies -
Description
Rabbit polyclonal to hnRNP K -
Host species
Rabbit -
Specificity
This antibody does not appear to react with isoform b of human hnRNPK (NP_112552.1). -
Tested applications
Suitable for: WB, IP, ICC/IF, IHC-P, ChIP/Chipmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Guinea pig, Cow, Rhesus monkey, Gorilla, Orangutan, Platypus, Elephant -
Immunogen
Synthetic peptide corresponding to a sequence from the C-terminus of isoform a of human hnRNP K. (NP_002131.2)
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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ChIP Related Products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab70492 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 51 kDa).
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IP |
Use at 1-4 µg/mg of lysate.
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ICC/IF |
Use at an assay dependent concentration.
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IHC-P |
Use at an assay dependent concentration.
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ChIP/Chip |
Use at an assay dependent concentration. PubMed: 20200201
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Notes |
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WB
1/2000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 51 kDa). |
IP
Use at 1-4 µg/mg of lysate. |
ICC/IF
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
ChIP/Chip
Use at an assay dependent concentration. PubMed: 20200201 |
Target
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Function
One of the major pre-mRNA-binding proteins. Binds tenaciously to poly(C) sequences. Likely to play a role in the nuclear metabolism of hnRNAs, particularly for pre-mRNAs that contain cytidine-rich sequences. Can also bind poly(C) single-stranded DNA. -
Sequence similarities
Contains 3 KH domains. -
Post-translational
modificationsArg-296 and Arg-299 are dimethylated, probably to asymmetric dimethylarginine. -
Cellular localization
Cytoplasm. Nucleus > nucleoplasm. In case of ASFV infection, there is a shift in the localization which becomes predominantly nuclear. - Information by UniProt
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Database links
- Entrez Gene: 528135 Cow
- Entrez Gene: 3190 Human
- Entrez Gene: 15387 Mouse
- Entrez Gene: 117282 Rat
- Entrez Gene: 709112 Rhesus monkey
- Omim: 600712 Human
- SwissProt: Q3T0D0 Cow
- SwissProt: P61978 Human
see all -
Alternative names
- CSBP antibody
- dC stretch binding protein antibody
- FLJ41122 antibody
see all
Images
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All lanes : Anti-hnRNP K antibody (ab70492) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Predicted band size: 51 kDa
Observed band size: 51,55 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma (left) and mouse squamous cell carcinoma (right) tissues labelling hnRNP K with ab70492 at 1/1000 (0.2µg/ml). Detection: DAB.
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IHC image of hnRNP K staining in Human Colon Adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab70492, 0.5 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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Detection of Human hnRNP K by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg/IP, 20% of IP loaded) using ab70492 at 3 µg/mg for IP and at 1.0 µg/ml for subsequent WB detection. Lane 2 represents IP IgG control.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (12)
ab70492 has been referenced in 12 publications.
- Avar M et al. An arrayed genome-wide perturbation screen identifies the ribonucleoprotein Hnrnpk as rate-limiting for prion propagation. EMBO J 41:e112338 (2022). PubMed: 36254605
- Xu W et al. SnoRD126 promotes the proliferation of hepatocellular carcinoma cells through transcriptional regulation of FGFR2 activation in combination with hnRNPK. Aging (Albany NY) 13:13300-13317 (2021). PubMed: 33891563
- Richter WF et al. Non-canonical H3K79me2-dependent pathways promote the survival of MLL-rearranged leukemia. Elife 10:N/A (2021). PubMed: 34263728
- Kwon PK et al. hnRNP K supports the maintenance of RORγ circadian rhythm through ERK signaling. FASEB J 35:e21507 (2021). PubMed: 33724572
- Xu C et al. A novel ZRS variant causes preaxial polydactyly type I by increased sonic hedgehog expression in the developing limb bud. Genet Med 22:189-198 (2020). PubMed: 31395945