Recombinant
RabMAb

Anti-hnRNP K antibody [EP943Y] (ab52600)

Overview

  • Product name
    Anti-hnRNP K antibody [EP943Y]
    See all hnRNP K primary antibodies
  • Description
    Rabbit monoclonal [EP943Y] to hnRNP K
  • Specificity
    This antibody detects both phosphorylated and unphosphorylated hnRNP K.
  • Tested applications
    Suitable for: WB, IP, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues near the N-terminus of human hnRNP.

  • Positive control
    • Jurkat cell lysate; HeLa cells; Human breast carcinoma tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Alternative versions available:

    Anti-hnRNP K antibody (Alexa Fluor® 488) [EP943Y] (ab203598)

    Anti-hnRNP K antibody (Alexa Fluor® 647) [EP943Y] (ab203599)

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EP943Y
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab52600 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100000. Detects a band of approximately 60 kDa (predicted molecular weight: 51 kDa).
IP 1/50.
IHC-P Use at an assay dependent concentration.
ICC/IF 1/100 - 1/250.

Target

  • Function
    One of the major pre-mRNA-binding proteins. Binds tenaciously to poly(C) sequences. Likely to play a role in the nuclear metabolism of hnRNAs, particularly for pre-mRNAs that contain cytidine-rich sequences. Can also bind poly(C) single-stranded DNA.
  • Sequence similarities
    Contains 3 KH domains.
  • Post-translational
    modifications
    Arg-296 and Arg-299 are dimethylated, probably to asymmetric dimethylarginine.
  • Cellular localization
    Cytoplasm. Nucleus > nucleoplasm. In case of ASFV infection, there is a shift in the localization which becomes predominantly nuclear.
  • Information by UniProt
  • Database links
  • Alternative names
    • CSBP antibody
    • dC stretch binding protein antibody
    • FLJ41122 antibody
    • Heterogeneous nuclear ribonucleoprotein K antibody
    • hnRNP K antibody
    • HNRNPK antibody
    • HNRPK antibody
    • HNRPK_HUMAN antibody
    • Transformation up regulated nuclear protein antibody
    • Transformation up-regulated nuclear protein antibody
    • Transformation upregulated nuclear protein antibody
    • TUNP antibody
    see all

Anti-hnRNP K antibody [EP943Y] images

  • Anti-hnRNP K antibody [EP943Y] (ab52600) at 1/100000 dilution + Jurkat cell lysate at 10 µg

    Secondary
    HRP-labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size : 51 kDa
    Observed band size : ~60 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemical staining of paraffin-embedded human breast carcinoma tissue using ab52600 at a dilution of 1/100-1/250.
  • Fluorescent immunostaining of HeLa cells using ab52600 at a dilution of 1/100-1/250.
  • ab52600 at 1/100 immunoprecipitating hnRNP K in HeLa whole cell lysate.

    For western blotting, a primary antibody was used at 1/1000 and a HRP-conjugated goat-anti-rabbit IgG was used as the secondary antibody (1/1000).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

References for Anti-hnRNP K antibody [EP943Y] (ab52600)

This product has been referenced in:
  • Sokolina K  et al. Systematic protein-protein interaction mapping for clinically relevant human GPCRs. Mol Syst Biol 13:918 (2017). IP . Read more (PubMed: 28298427) »
  • Seo J  et al. Oxidative Stress Triggers Body-Wide Skipping of Multiple Exons of the Spinal Muscular Atrophy Gene. PLoS One 11:e0154390 (2016). WB . Read more (PubMed: 27111068) »

See all 17 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Normal colon)
Specification
Normal colon
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric buffer, pH6
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
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Verified customer

Submitted May 31 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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