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Product name Anti-hnRNP K antibody [EP943Y] See all hnRNP K primary antibodies
Rabbit monoclonal [EP943Y] to hnRNP K
Specificity This antibody detects both phosphorylated and unphosphorylated hnRNP K.
Tested applications Suitable for:
WB, IP, IHC-P, ICC/IF more details
Mouse, Rat, Human
A synthetic peptide corresponding to residues near the N-terminus of human hnRNP.
Jurkat cell lysate; HeLa cells; Human breast carcinoma tissue.
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb
® technology. RabMAb ® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
Alternative versions available:
Anti-hnRNP K antibody (Alexa Fluor® 488) [EP943Y] ( ab203598) Anti-hnRNP K antibody (Alexa Fluor® 647) [EP943Y] ( ab203599)
Storage instructions Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purity Tissue culture supernatant
Clone number EP943Y
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100000. Detects a band of approximately 60 kDa (predicted molecular weight: 51 kDa).
Use at an assay dependent concentration.
1/100 - 1/250.
Function One of the major pre-mRNA-binding proteins. Binds tenaciously to poly(C) sequences. Likely to play a role in the nuclear metabolism of hnRNAs, particularly for pre-mRNAs that contain cytidine-rich sequences. Can also bind poly(C) single-stranded DNA.
Sequence similarities Contains 3 KH domains.
Post-translational modifications Arg-296 and Arg-299 are dimethylated, probably to asymmetric dimethylarginine.
Cellular localization Cytoplasm. Nucleus > nucleoplasm. In case of ASFV infection, there is a shift in the localization which becomes predominantly nuclear.
Information by UniProt
dC stretch binding protein antibody
Anti-hnRNP K antibody [EP943Y] images
Western blot - hnRNP K antibody [EP943Y] (ab52600)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP K antibody [EP943Y] (ab52600)
Immunohistochemical staining of paraffin-embedded human breast carcinoma tissue using ab52600 at a dilution of 1/100-1/250.
Immunocytochemistry/ Immunofluorescence - hnRNP K antibody [EP943Y] (ab52600)
Fluorescent immunostaining of HeLa cells using ab52600 at a dilution of 1/100-1/250.
Immunoprecipitation - Anti-hnRNP K antibody [EP943Y] (ab52600)
ab52600 at 1/100 immunoprecipitating hnRNP K in HeLa whole cell lysate.
For western blotting, a primary antibody was used at 1/1000 and a HRP-conjugated goat-anti-rabbit IgG was used as the secondary antibody (1/1000).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
References for Anti-hnRNP K antibody [EP943Y] (ab52600)
This product has been referenced in:
et al. Quantitative proteomics identifies host factors modulated during acute hepatitis E virus infection in the swine model. J Virol 89:129-43 (2015).
Read more (PubMed: 25320303) »
et al. Regulation of C-X-C chemokine gene expression by keratin 17 and hnRNP K in skin tumor keratinocytes. J Cell Biol 208:613-27 (2015).
Read more (PubMed: 25713416) »
See all 11 Publications
for this product
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"