The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100000. Detects a band of approximately 60 kDa (predicted molecular weight: 51 kDa).
Use at an assay dependent concentration.
1/100 - 1/250.
FunctionOne of the major pre-mRNA-binding proteins. Binds tenaciously to poly(C) sequences. Likely to play a role in the nuclear metabolism of hnRNAs, particularly for pre-mRNAs that contain cytidine-rich sequences. Can also bind poly(C) single-stranded DNA.
Sequence similaritiesContains 3 KH domains.
Post-translational modificationsArg-296 and Arg-299 are dimethylated, probably to asymmetric dimethylarginine.
Cellular localizationCytoplasm. Nucleus > nucleoplasm. In case of ASFV infection, there is a shift in the localization which becomes predominantly nuclear.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP K antibody [EP943Y] (ab52600)
Immunohistochemical staining of paraffin-embedded human breast carcinoma tissue using ab52600 at a dilution of 1/100-1/250.
Immunocytochemistry/ Immunofluorescence - hnRNP K antibody [EP943Y] (ab52600)
Fluorescent immunostaining of HeLa cells using ab52600 at a dilution of 1/100-1/250.
Immunoprecipitation - Anti-hnRNP K antibody [EP943Y] (ab52600)
ab52600 at 1/100 immunoprecipitating hnRNP K in HeLa whole cell lysate.
For western blotting, a primary antibody was used at 1/1000 and a HRP-conjugated goat-anti-rabbit IgG was used as the secondary antibody (1/1000).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
References for Anti-hnRNP K antibody [EP943Y] (ab52600)
This product has been referenced in:
Rogée S et al. Quantitative proteomics identifies host factors modulated during acute hepatitis E virus infection in the swine model. J Virol89:129-43 (2015).
Read more (PubMed: 25320303) »
Vickers TA & Crooke ST Antisense oligonucleotides capable of promoting specific target mRNA reduction via competing RNase H1-dependent and independent mechanisms. PLoS One9:e108625 (2014).
Read more (PubMed: 25299183) »