Recombinant
RabMAb

Anti-hnRNP K antibody [EPR944] (ab134060)

Overview

  • Product name
    Anti-hnRNP K antibody [EPR944]
    See all hnRNP K primary antibodies
  • Description
    Rabbit monoclonal [EPR944] to hnRNP K
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) (internal sequence)

  • Positive control
    • Jurkat and A549 cell lysates; Human breast carcinoma tissue.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    pH: 7.40
    Preservative: 0.01% Sodium azide
    Constituents: 50% Glycerol, 0.05% BSA
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR944
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab134060 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50000 - 1/200000. Predicted molecular weight: 50 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IP.
  • Target

    • Function
      One of the major pre-mRNA-binding proteins. Binds tenaciously to poly(C) sequences. Likely to play a role in the nuclear metabolism of hnRNAs, particularly for pre-mRNAs that contain cytidine-rich sequences. Can also bind poly(C) single-stranded DNA.
    • Sequence similarities
      Contains 3 KH domains.
    • Post-translational
      modifications
      Arg-296 and Arg-299 are dimethylated, probably to asymmetric dimethylarginine.
    • Cellular localization
      Cytoplasm. Nucleus > nucleoplasm. In case of ASFV infection, there is a shift in the localization which becomes predominantly nuclear.
    • Information by UniProt
    • Database links
    • Alternative names
      • CSBP antibody
      • dC stretch binding protein antibody
      • FLJ41122 antibody
      • Heterogeneous nuclear ribonucleoprotein K antibody
      • hnRNP K antibody
      • HNRNPK antibody
      • HNRPK antibody
      • HNRPK_HUMAN antibody
      • Transformation up regulated nuclear protein antibody
      • Transformation up-regulated nuclear protein antibody
      • Transformation upregulated nuclear protein antibody
      • TUNP antibody
      see all

    Images

    • All lanes : Anti-hnRNP K antibody [EPR944] (ab134060) at 1/50000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : A549 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 50 kDa

    • Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling hnRNP K with ab134060 at 1/100 dilution.

    References

    ab134060 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab134060.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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