Anti-hnRNP K (phospho S284) antibody (ab74066)

Overview

  • Product nameAnti-hnRNP K (phospho S284) antibody
    See all hnRNP K primary antibodies
  • Description
    Rabbit polyclonal to hnRNP K (phospho S284)
  • Specificityab74066 detects endogenous levels of hnRNP K only when phosphorylated at serine 284.
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic phosphopeptide derived from human hnRNP K around the phosphorylation site of serine 284 (D-M-SP-P-R).

  • Positive control
    • Extracts from 3T3 cells treated with EGF (200ng/ml, 30mins).

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab74066 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab74066 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).
ELISA 1/20000.

Target

  • FunctionOne of the major pre-mRNA-binding proteins. Binds tenaciously to poly(C) sequences. Likely to play a role in the nuclear metabolism of hnRNAs, particularly for pre-mRNAs that contain cytidine-rich sequences. Can also bind poly(C) single-stranded DNA.
  • Sequence similaritiesContains 3 KH domains.
  • Post-translational
    modifications
    Arg-296 and Arg-299 are dimethylated, probably to asymmetric dimethylarginine.
  • Cellular localizationCytoplasm. Nucleus > nucleoplasm. In case of ASFV infection, there is a shift in the localization which becomes predominantly nuclear.
  • Information by UniProt
  • Database links
  • Alternative names
    • CSBP antibody
    • dC stretch binding protein antibody
    • FLJ41122 antibody
    • Heterogeneous nuclear ribonucleoprotein K antibody
    • hnRNP K antibody
    • HNRNPK antibody
    • HNRPK antibody
    • HNRPK_HUMAN antibody
    • Transformation up regulated nuclear protein antibody
    • Transformation up-regulated nuclear protein antibody
    • Transformation upregulated nuclear protein antibody
    • TUNP antibody
    see all

Anti-hnRNP K (phospho S284) antibody images

  • All lanes : Anti-hnRNP K (phospho S284) antibody (ab74066) at 1/500 dilution

    Lane 1 : Extracts from 3T3 cells treated with EGF (200ng/ml, 30mins)
    Lane 2 : Extracts from 3T3 cells treated with EGF (200ng/ml, 30mins) with immunising phosphopeptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 51 kDa
    Observed band size : 51 kDa

References for Anti-hnRNP K (phospho S284) antibody (ab74066)

ab74066 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"