Overview

  • Product name
  • Description
    Rabbit polyclonal to hnRNP L
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Chimpanzee, Ferret, Non human primates, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region within amino acids 350-400 of Human hnRNP L (NP_001524.2).

  • Positive control
    • 293T, HeLa and Jurkat whole cell lysates; mouse NIH 3T3 whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab156682 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 64 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function
    This protein is a component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. Is associated with most nascent transcripts including those of the landmark giant loops of amphibian lampbrush chromosomes. Associates, together with APEX1, to the negative calcium responsive element (nCaRE) B2 of the APEX2 promoter.
  • Sequence similarities
    Contains 3 RRM (RNA recognition motif) domains.
  • Post-translational
    modifications
    Several isoelectric forms of the L protein are probably the results of post-translational modifications.
  • Cellular localization
    Nucleus > nucleoplasm. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
  • Information by UniProt
  • Database links
  • Alternative names
    • D830027H13Rik antibody
    • FLJ35509 antibody
    • Heterogeneous nuclear ribonucleoprotein L antibody
    • hnRNP L antibody
    • hnRNP-L antibody
    • Hnrnpl antibody
    • hnRPL antibody
    • HNRPL_HUMAN antibody
    • P/OKcl.14 antibody
    see all

Images

  • All lanes : Anti-hnRNP L antibody (ab156682) at 0.1 µg/ml

    Lane 1 : 293T whole cell lysate at 50 µg
    Lane 2 : 293T whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 50 µg
    Lane 4 : Jurkat whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 64 kDa


    Exposure time: 30 seconds
  • All lanes : Anti-hnRNP L antibody (ab156682) at 0.4 µg/ml

    Lane 1 : Mouse NIH 3T3 whole cell lysate at 50 µg
    Lane 2 : Mouse NIH 3T3 whole cell lysate at 15 µg

    Developed using the ECL technique.

    Predicted band size: 64 kDa


    Exposure time: 10 seconds
  • Detection of hnRNP L by Western blot of Immunoprecipitate
    Lane 1: Immunoprecipitation of 293T whole cell lysate using ab156682 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Lane 2: lysate is precipitated with an antibody to another region of hnRNP L.
    Lane 3: lysate is precipitated using an IgG control antibody.
    Detection: using ab156682 at 1 µg/ml.

References

ab156682 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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