![Immunocytochemistry/ Immunofluorescence - hnRNP M1-M4 antibody [2A6] (ab9548) image](#ab9548_1.jpg)
![Western blot - hnRNP M1-M4 antibody [2A6] (ab9548) image](#ab9548_2.jpg)
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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> RNA Processing
Overview
Product name
Anti-hnRNP M3-M4 antibody [2A6]
See all hnRNP M3-M4 products (4) ...
Description
Mouse monoclonal [2A6] to hnRNP M3-M4
Specificity
This antibody is specific for human RNP M3-M4.
Tested applications
IF, IP, ICC/IF, WBmore details
Cross reactivity
Reacts with
Mouse, Rat, Rabbit, Cow, Human, Pig
Immunogen
M19 fusion protein containing full-lengthhuman protein.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: Ascites
Concentration
Concentration information loading...
Purity
Ascites
Clonality
Monoclonal
Clone number
2A6
Isotype
IgG2b
Light chain type
kappa
Research areas
Epigenetics and Nuclear Signaling >> Chromatin Binding Proteins >> DNA / RNA binding
Epigenetics and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Splicing
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA / Nucleotides
Epigenetics and Nuclear Signaling >> DNA / RNA >> RNA Processing
Applications
Show applications key
Our Abpromise guarantee covers the use of ab9548 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IF
IP
Application notes
IF: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
WB: 1/1000. Predicted molecular weight: 77 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Pre-mRNA binding protein in vivo, binds avidly to poly(G) and poly(U) RNA homopolymers in vitro. Involved in splicing. Acts as a receptor for carcinoembryonic antigen in Kupffer cells, may initiate a series of signaling events leading to tyrosine phosphorylation of proteins and induction of IL-1 alpha, IL-6, IL-10 and tumor necrosis factor alpha cytokines.
Sequence similarities
Contains 3 RRM (RNA recognition motif) domains.
Post-translational
modifications
Sumoylated.
Cellular localization
Nucleus > nucleolus.
Target information above from: UniProt accessionP52272
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Heterogeneous nuclear ribonucleoprotein M antibody
- Heterogenous nuclear ribonucleoprotein M4 antibody
- hnRNA binding protein M4 antibody
see all
Database links
- Entrez Gene: 515290 Cow
- Entrez Gene: 4670 Human
- Entrez Gene: 76936 Mouse
- Entrez Gene: 116655 Rat
- Omim: 160994 Human
- SwissProt: P52272 Human
- SwissProt: Q9D0E1 Mouse
- SwissProt: Q62826 Rat
see all
Anti-hnRNP M3-M4 antibody [2A6] images:
Immunocytochemistry/ Immunofluorescence - hnRNP M1-M4 antibody [2A6] (ab9548)
![Immunocytochemistry/ Immunofluorescence - hnRNP M1-M4 antibody [2A6] (ab9548)](/ps/datasheet/Images/9/ab9548/ab9548_1.jpg)
ab9548 at 1/1000 dilution staining human U2OS osteosarcoma cells by ICC/IF. The cells were paraformaldehyde fixed and blocked with BSA prior to incubation with ab9548. A FITC conjugated goat anti-mouse antibody was used as the secondary.
This image is courtesy of an Abreview submitted by Dr Stoil Dmitrov
Western blot - hnRNP M1-M4 antibody [2A6] (ab9548)
![Western blot - hnRNP M1-M4 antibody [2A6] (ab9548)](/ps/datasheet/Images/9/ab9548/ab9548_2.jpg)
Anti-hnRNP M3-M4 antibody [2A6] (ab9548) at 1/10000 dilution + 293T cells whole cell lysate at 30 µg
Secondary
HRP conjugated Sheep anti-mouse IgG
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 77 kDa
Observed band size : 70 kDa (why is the actual band size different from the predicted?)
Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 10 seconds
This image is courtesy of an Abreview submitted by Dr Stoil Dmitrov
References for Anti-hnRNP M3-M4 antibody [2A6] (ab9548)
This product has been referenced in:
- Datar KVet al. The human hnRNP M proteins: identification of a methionine/arginine-rich repeat motif in ribonucleoproteins. Nucleic Acids Res 21:439-46 (1993).Read more (PubMed: 8441656) »
See 1 publication for this product
Publishing research using ab9548? Please let us know so that we can cite the reference in this datasheet
![hnRNP M1-M4 antibody [2A6] for ICC/IF in Human (9548)](/ps/Reviews/Images/ab7276_29663.jpg)
![hnRNP M1-M4 antibody [2A6] for WB in Human (9548)](/ps/Reviews/Images/ab7275_29664.jpg)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"