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Rabbit polyclonal to hnRNP M3-M4
Recombinant fragment, corresponding to a region within amino acids 1-180 of Human hnRNP M3-M4 (P52272).
Human colon carcinoma tissue; HeLa cells; 293T and HeLa whole cell lysates.
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.01% Thimerosal (merthiolate) Constituents: 0.75% Glycine, 1.21% Tris, 20% Glycerol
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/5000 - 1/20000. Predicted molecular weight: 77 kDa.
1/100 - 1/1000.
1/100 - 1/1000.
Pre-mRNA binding protein in vivo, binds avidly to poly(G) and poly(U) RNA homopolymers in vitro. Involved in splicing. Acts as a receptor for carcinoembryonic antigen in Kupffer cells, may initiate a series of signaling events leading to tyrosine phosphorylation of proteins and induction of IL-1 alpha, IL-6, IL-10 and tumor necrosis factor alpha cytokines.
Contains 3 RRM (RNA recognition motif) domains.
Information by UniProt
CEA receptor antibody
Heterogeneous nuclear ribonucleoprotein M antibody
Western blot - Anti-hnRNP M3-M4 antibody (ab126103)
All lanes : Anti-hnRNP M3-M4 antibody (ab126103) at 1/10000 dilution Lane 1 : 293T whole cell lysate Lane 2 : HeLa whole cell lysate Lysates/proteins at 30 µg per lane. Predicted band size: 77 kDa
Immunocytochemistry/ Immunofluorescence - Anti-hnRNP M3-M4 antibody (ab126103)
ab126103, at 1/500 dilution, staining hnRNP M3-M4 in paraformaldehyde-fixed HeLa cells by Immunofluorescence (green). Alpha-tubulin filaments are stained in red and nuclei in blue.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP M3-M4 antibody (ab126103)
ab126103, at 1/500 dilution, staining hnRNP M3-M4 in paraffin-embedded Human colon carcinoma tissue by Immunohistochemistry.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"