Overview

  • Product nameAnti-hnRNP M3-M4 antibody
    See all hnRNP M3-M4 primary antibodies
  • Description
    Mouse monoclonal to hnRNP M3-M4
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment: KMEEESGAPG VPSGNGAPGP KGEGERPAQN EKRKEKNIKR GGNRFEPYAN PTKRYRAFIT NIPFDVKWQS LKDLVKEKVG EVTYVELLMD AEGKSR, corresponding to amino acids 17-113 of Human hnRNP M3-M4

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • IsotypeIgG2a
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab55486 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 77 kDa.
IHC-P Use a concentration of 3 µg/ml.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • FunctionPre-mRNA binding protein in vivo, binds avidly to poly(G) and poly(U) RNA homopolymers in vitro. Involved in splicing. Acts as a receptor for carcinoembryonic antigen in Kupffer cells, may initiate a series of signaling events leading to tyrosine phosphorylation of proteins and induction of IL-1 alpha, IL-6, IL-10 and tumor necrosis factor alpha cytokines.
  • Sequence similaritiesContains 3 RRM (RNA recognition motif) domains.
  • Post-translational
    modifications
    Sumoylated.
  • Cellular localizationNucleus, nucleolus.
  • Information by UniProt
  • Database links
  • Alternative names
    • CEA receptor antibody
    • CEAR antibody
    • Heterogeneous nuclear ribonucleoprotein M antibody
    • Heterogenous nuclear ribonucleoprotein M4 antibody
    • hnRNA binding protein M4 antibody
    • hnRNP M antibody
    • Hnrnpm antibody
    • HNRNPM4 antibody
    • HNRPM antibody
    • HNRPM_HUMAN antibody
    • HNRPM4 antibody
    • HTGR1 antibody
    • M3 antibody
    • M4 antibody
    • M4 protein antibody
    • N-acetylglucosamine receptor 1 antibody
    • NAGR1 antibody
    see all

Anti-hnRNP M3-M4 antibody images

  • hnRNP M3-M4 antibody (ab55486) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human thyroid nodular goiter.


  • Predicted band size : 77 kDa
    hnRNP M3-M4 antibody (ab55486) at 1ug/lane + HepG2 cell lysate at 25ug/lane.
  • ab55486 at 10 ug/ml staining HNRPM in human HepG2 cells by Immunocytochemistry/ Immunofluorescence.

References for Anti-hnRNP M3-M4 antibody (ab55486)

ab55486 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunoprecipitation
Sample Human Cell lysate - whole cell (293 cell total extract)
Total protein in input 1000 µg
Specification 293 cell total extract
Immuno-precipitation step Protein A
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Submitted Oct 17 2011

Application Western blot
Sample Human Cell lysate - whole cell (HCT116 cells)
Loading amount 50 µg
Specification HCT116 cells
Gel Running Conditions Reduced Non-Denaturing (Native) (6-15% gradient gel)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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Verified customer

Submitted Oct 04 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"