Overview

  • Product nameAnti-hnRNP U antibody
    See all hnRNP U primary antibodies
  • Description
    Rabbit polyclonal to hnRNP U
  • Tested applicationsSuitable for: WB, IP, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat
    Predicted to work with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 614-800 of Mouse hnRNP U fused to MBT.

  • Positive control
    • Lysates of SAOS2 cells transfected with pRc/CMV vector expressing Myc tagged hnRNP U; Mouse P19 neurons.

Properties

Applications

Our Abpromise guarantee covers the use of ab122906 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/3000. Predicted molecular weight: 91 kDa.
IP Use at an assay dependent dilution.
ICC/IF 1/500 - 1/1000.

Target

  • FunctionComponent of the CRD-mediated complex that promotes MYC mRNA stabilization. Binds to pre-mRNA. Has high affinity for scaffold-attached region (SAR) DNA. Binds to double- and single-stranded DNA and RNA.
  • Sequence similaritiesContains 1 B30.2/SPRY domain.
    Contains 1 SAP domain.
  • Post-translational
    modifications
    Extensively phosphorylated.
    Arg-733 and Arg-739 are dimethylated, probably to asymmetric dimethylarginine.
  • Cellular localizationNucleus. Cytoplasm. Cell surface. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Component of ribonucleosomes. Also found associated with the cell surface.
  • Information by UniProt
  • Database links
  • Alternative names
    • Heterogeneous nuclear ribonucleoprotein U antibody
    • hnRNP U antibody
    • hnRNP U protein antibody
    • HNRNPU antibody
    • hnRNPU protein antibody
    • HNRPU antibody
    • HNRPU_HUMAN antibody
    • p120 antibody
    • p120 nuclear protein antibody
    • pp120 antibody
    • SAF A antibody
    • SAF-A antibody
    • SAFA antibody
    • Scaffold attachment factor A antibody
    • U21.1 antibody
    see all

Anti-hnRNP U antibody images

  • Lanes 1 - 2 : anti Myc antibody
    Lanes 3 - 4 : Anti-hnRNP U antibody (ab122906) at 1/1000 dilution

    Lane 1 : lysates from SAOS2 cells transfected with pRc/CMV vector
    Lane 2 : lysates from SAOS2 cells transfected with pRc/CMV vector expressing Myc tagged hnRNP U
    Lane 3 : lysates from SAOS2 cells transfected with pRc/CMV vector
    Lane 4 : lysates from SAOS2 cells transfected with pRc/CMV vector expressing Myc tagged hnRNP U


    Predicted band size : 91 kDa
  • Immunofuorescent analysis of hnRNP U expression using ab122906 in Mouse P19 neurons (left) and in cells with in situ extracted nuclear matrix (right). Note that hnRNP U is concentrated in intranuclear speckles throughout the nucleoplasm.

References for Anti-hnRNP U antibody (ab122906)

This product has been referenced in:
  • Taniura H & Yoshikawa K Necdin interacts with the ribonucleoprotein hnRNP U in the nuclear matrix. J Cell Biochem 84:545-55 (2002). Read more (PubMed: 11813259) »

See 1 Publication for this product

Product Wall

Thank you for your message which has been forwarded to me as my colleague Karin is currently away from the office.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I ...

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Thank you for that information. I now understand more fully the situation.

I am sorry that ab122906 has not been performing as expected. I cannot explain this or the fluorescent yellow colour you have described. I have just been to have a loo...

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Thank you for getting back to me with that information. This will be of great help to us.

From the protocol provided I have a few further questions if you wouldn't mind:

1. You mention that "no longer works in WB or IP". D...

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I am very sorry for that mistake. I was under the impression that it was Western blotting that you had been having difficulty with with this antibody? I have now attached the alternative questionnaire.

Have you tried Western blotting with the...

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Thank you for getting back to me.

I am sorry to hear that the antibody has not been working in Western blotting. I'd be happy to provide you with a replacement antibody if you would like. Unfortunately, we do not have an alternative lot of ab...

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Thank you for contacting us and reporting the problems you have observed with the Anti-hnRNP U antibody (ab122906).

I have looked into this and I can reassure you that the antibody was not in transit for 10 days but only the usualone. The pro...

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