ICC/IF image of ab66661 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab66661, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human heart tissue labelling HNRNPA0 with ab6661 at 4-8µg/ml. Arrows indicate positively labelled myocardial cells. Magnification: 400X.
References for Anti-HNRNPA0 antibody (ab66661)
This product has been referenced in:
Naji S et al. Host cell interactome of HIV-1 Rev includes RNA helicases involved in multiple facets of virus production. Mol Cell Proteomics11:M111.015313 (2012).
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Ranjbarvaziri S et al. Quantum dot labeling using positive charged peptides in human hematopoetic and mesenchymal stem cells. Biomaterials32:5195-205 (2011).
Read more (PubMed: 21549422) »