• Product nameAnti-HNRNPA0 antibody
    See all HNRNPA0 primary antibodies
  • Description
    Rabbit polyclonal to HNRNPA0
  • Tested applicationsSuitable for: WB, IHC-P, IHC-FoFr, ICC/IF, ELISAmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Cow, Cat, Dog, Pig
  • Immunogen

    A region within synthetic peptide: YFQNHDAADK AAVVKFHPIQ GHRVEVKKAV PKEDIYSGGG GGGSRSSRGG, corresponding to internal sequence amino acids 145-194 of Human HNRNPA0

  • Positive control
    • Human heart and liver tissue sections and Jurkat cell lysate



Our Abpromise guarantee covers the use of ab66661 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.625 µg/ml. Detects a band of approximately 34, 36 kDa (predicted molecular weight: 31 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.
IHC-FoFr Use at an assay dependent concentration. PubMed: 21970342
ICC/IF Use a concentration of 1 µg/ml.
ELISA Use at an assay dependent concentration. ELISA titre using peptide based assay 1:312500.


Anti-HNRNPA0 antibody images

  • Anti-HNRNPA0 antibody (ab66661) at 0.625 µg/ml + Jurkat cell lysate at 10 µg

    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 31 kDa
    Observed band size : 34 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 36 kDa. We are unsure as to the identity of these extra bands.
  • ICC/IF image of ab66661 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab66661, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling HNRNPA0 with ab66661 at 4-8µg/ml. Arrows indicate positively labelled hepatocyte cells. Magnification: 400X.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human heart tissue labelling HNRNPA0 with ab6661 at 4-8µg/ml. Arrows indicate positively labelled myocardial cells. Magnification: 400X.

References for Anti-HNRNPA0 antibody (ab66661)

This product has been referenced in:
  • Naji S  et al. Host cell interactome of HIV-1 Rev includes RNA helicases involved in multiple facets of virus production. Mol Cell Proteomics 11:M111.015313 (2012). WB . Read more (PubMed: 22174317) »
  • Ranjbarvaziri S  et al. Quantum dot labeling using positive charged peptides in human hematopoetic and mesenchymal stem cells. Biomaterials 32:5195-205 (2011). IHC-P ; Human . Read more (PubMed: 21549422) »

See all 3 Publications for this product

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