Overview

  • Product nameAnti-HNRPLL antibody
    See all HNRPLL primary antibodies
  • Description
    Rabbit polyclonal to HNRPLL
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rabbit, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide: EGENRREATP RGGGDGGGGG RSFSQPEAGG SHHKVSVSPV VHVRGLCESV , corresponding to amino acids 38-87 of Human HNRPLL

  • Positive control
    • HepG2 cell lysate, human lung and heart tissue.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab66775 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

Target

Anti-HNRPLL antibody images

  • All lanes : Anti-HNRPLL antibody (ab66775) at 2.5 µg/ml

    Lane 1 : HepG2 cell lysate
    Lane 2 : HepG2 cell lysate with blocking peptide at 2 µg/ml

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 60 kDa
  • Immunohistochemical analysis of HNRPLL expression in paraffin embedded human lung using 4µg/ml ab66775. Arrows indicate positively labelled alveolar cells.

  • All lanes : Anti-HNRPLL antibody (ab66775) at 1.25 µg/ml

    Lane 1 : marker
    Lane 2 : HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 60 kDa
    Observed band size : 60 kDa
    Additional bands at : 68 kDa. We are unsure as to the identity of these extra bands.
    gel concentration: 12%
  • Immunohistochemical analysis of HNRPLL expression in paraffin embedded human heart using 4µg/ml ab66775. Arrows indicate positively labelled myocardial cells.

References for Anti-HNRPLL antibody (ab66775)

ab66775 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab66775.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"