Overview

  • Product name
  • Description
    Rabbit polyclonal to HNRPLL
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rabbit, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide: EGENRREATP RGGGDGGGGG RSFSQPEAGG SHHKVSVSPV VHVRGLCESV , corresponding to amino acids 38-87 of Human HNRPLL

  • Positive control
    • HepG2 cell lysate, human lung and heart tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab66775 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

Target

Images

  • All lanes : Anti-HNRPLL antibody (ab66775) at 2.5 µg/ml

    Lane 1 : HepG2 cell lysate
    Lane 2 : HepG2 cell lysate with blocking peptide at 2 µg/ml

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 60 kDa
  • Immunohistochemical analysis of HNRPLL expression in paraffin embedded human lung using 4µg/ml ab66775. Arrows indicate positively labelled alveolar cells.

  • All lanes : Anti-HNRPLL antibody (ab66775) at 1.25 µg/ml

    Lane 1 : marker
    Lane 2 : HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 60 kDa
    Observed band size : 60 kDa
    Additional bands at : 68 kDa. We are unsure as to the identity of these extra bands.gel concentration: 12%
  • Immunohistochemical analysis of HNRPLL expression in paraffin embedded human heart using 4µg/ml ab66775. Arrows indicate positively labelled myocardial cells.

References

ab66775 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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