Mouse, Human Predicted to work with:
Chimpanzee, Rhesus monkey, Orangutan
Synthetic peptide, corresponding to a region between residues 50 and 100 of human HNRPUL1 (NP_008971.2).
Human controls: Breast Carcinoma, Colon Carcinoma, Laryngeal Squamous Cell Carcinoma, Linitis Plastica Stomach Cancer, Non Small Cell Lung Cancer, Ovarian Carcinoma, Prostate Carcinoma, Skin Basal Cell Carcinoma, Small Cell Lung Cancer, Stomach Adenocarcinoma, Testicular Seminoma
Mouse controls: Brain, Testis
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline
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Immunogen affinity purified
ab84774 was affinity purified using an epitope specific to HNRPUL1 immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/100 - 1/500.
Epitope exposure is recommended.
Epitope exposure with citrate or tris-EDTA pH9.0 buffer will enhance staining.
Likely to work with frozen sections.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Acts as a basic transcriptional regulator. Represses basic transcription driven by several virus and cellular promoters. When associated with BRD7, activates transcription of glucocorticoid-responsive promoter in the absence of ligand-stimulation. Plays also a role in mRNA processing and transport. Binds avidly to poly(G) and poly(C) RNA homopolymers in vitro.
Contains 1 B30.2/SPRY domain. Contains 1 SAP domain.
Staining of HNRPUL1 by Immunohistochemistry on a formalin fixed, paraffin embedded section of human breast carcinoma using a 1/100 dilution of ab84774. Detection: Red fluorescent goat anti rabbit IgG highly cross adsorbed antibody used at a dilution of 1/100.