Purification notesab84774 was affinity purified using an epitope specific to HNRPUL1 immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesIHC-P: 1/100 - 1/500.
Epitope exposure is recommended.
Epitope exposure with citrate or tris-EDTA pH9.0 buffer will enhance staining.
Likely to work with frozen sections.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionActs as a basic transcriptional regulator. Represses basic transcription driven by several virus and cellular promoters. When associated with BRD7, activates transcription of glucocorticoid-responsive promoter in the absence of ligand-stimulation. Plays also a role in mRNA processing and transport. Binds avidly to poly(G) and poly(C) RNA homopolymers in vitro.
Sequence similaritiesContains 1 B30.2/SPRY domain. Contains 1 SAP domain.
Staining of HNRPUL1 by Immunohistochemistry on a formalin fixed, paraffin embedded section of human breast carcinoma using a 1/100 dilution of ab84774. Detection: Red fluorescent goat anti rabbit IgG highly cross adsorbed antibody used at a dilution of 1/100.