Overview

  • Product name
    Anti-HNRPUL1 antibody [EP2633Y]
    See all HNRPUL1 primary antibodies
  • Description
    Rabbit monoclonal [EP2633Y] to HNRPUL1
  • Tested applications
    Suitable for: WB, IP, IHC-P, ICC, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues on the C-terminus of human HNRPUL1.

  • Positive control
    • HeLa cell lysate, HeLa cells and human brain tissue.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab68480 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 96 kDa (predicted molecular weight: 96 kDa).
IP 1/40.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC 1/100 - 1/250.
Flow Cyt 1/80. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Acts as a basic transcriptional regulator. Represses basic transcription driven by several virus and cellular promoters. When associated with BRD7, activates transcription of glucocorticoid-responsive promoter in the absence of ligand-stimulation. Plays also a role in mRNA processing and transport. Binds avidly to poly(G) and poly(C) RNA homopolymers in vitro.
  • Sequence similarities
    Contains 1 B30.2/SPRY domain.
    Contains 1 SAP domain.
  • Domain
    The RGG-box domain is methylated.
  • Post-translational
    modifications
    Methylated.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Adenovirus early region 1B associated protein 5 antibody
    • Adenovirus early region 1B-associated protein 5 antibody
    • E130317O14Rik antibody
    • E1B 55 kDa associated protein 5 antibody
    • E1B AP5 antibody
    • E1B-55 kDa-associated protein 5 antibody
    • E1B-AP5 antibody
    • E1BAP5 antibody
    • fb53d10 antibody
    • fk45c03 antibody
    • FLJ12944 antibody
    • Heterogeneous nuclear ribonucleoprotein U-like protein 1 antibody
    • HNRL1_HUMAN antibody
    • Hnrnpul1 antibody
    • HNRPUL1 antibody
    • MGC36621 antibody
    • MGC78330 antibody
    • wu:fb53d10 antibody
    • wu:fk45c03 antibody
    • zgc:85971 antibody
    see all

Images

  • Anti-HNRPUL1 antibody [EP2633Y] (ab68480) at 1/50000 dilution + HeLa cell lysate at 10 µg

    Secondary
    goat anti-rabbit, HRP conjugated at 1/2000 dilution

    Predicted band size : 96 kDa
    Observed band size : 96 kDa
    Additional bands at : 125 kDa. We are unsure as to the identity of these extra bands.
  • ab68480 at 1/100 dilution staining HNRPUL1 in HeLa cells by Immunocytochemistry.
  • ab68480 at 1/250 dilution staining HNRPUL1 in human brain by Immunohistochemistry, Paraffin-embedded tissue.
  • Overlay histogram showing HeLa cells stained with ab68480 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab68480, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References

This product has been referenced in:
  • Vickers TA & Crooke ST Antisense oligonucleotides capable of promoting specific target mRNA reduction via competing RNase H1-dependent and independent mechanisms. PLoS One 9:e108625 (2014). WB . Read more (PubMed: 25299183) »
  • Massimelli MJ  et al. Interplay between polyadenylate-binding protein 1 and Kaposi's sarcoma-associated herpesvirus ORF57 in accumulation of polyadenylated nuclear RNA, a viral long noncoding RNA. J Virol 87:243-56 (2013). Read more (PubMed: 23077296) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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