Anti-Holocytochrome C antibody (ab2542)

Overview

  • Product name
    Anti-Holocytochrome C antibody
  • Description
    Mouse monoclonal to Holocytochrome C
  • Specificity
    This antibody detects a 12 kDa protein corresponding to the apparent molecular weight of cytochrome c. The antibody is specific for holocytochrome c. It does not detect apocytochrome c.
  • Tested applications
    Suitable for: ICC/IF, IP, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Does not react with: Horse, Guinea pig, Cow, Pig
  • Immunogen

    Cytochrome c purified from human heart.

  • General notes


    The precursor of cytochrome c, apocytochrome c, is synthesized in the cytoplasm. Upon translocation into mitochondria, cytochrome c acquires a heme moiety required for functionality in the mitochondrial respiration chain. The heme-bound form of cytochrome c is called holocytochrome c. When released into the cytosol, cytochrome c can activate the caspases responsible for apoptosis.

Properties

Applications

Our Abpromise guarantee covers the use of ab2542 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 6 µg/ml.
IP Use at an assay dependent concentration.
WB Use a concentration of 5 - 10 µg/ml.

Images

  • ab2542 staining differentiating (rat) PC12 cells by ICC/IF.  Cells were PFA fixed and permeabilized in 0.2% Triton X100 for 5 minutes prior to blocking in 10% serum at RT for 1 hour.  The primary antibody was incubated with the sample for 1 hour at 6 µg/ml.  An Alexa Fluor® 488 conjugated goat anti-mouse antibody diluted 1/1000 was used as the secondary.  Cells were counterstained with the nuclear Topro-3 stain.

    See Abreview

References

ab2542 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (differentiating PC12 cells)
Specification
differentiating PC12 cells
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.2% Triton X100 for 5 minutes
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Username

Dr. Anne Chiaramello

Verified customer

Submitted Apr 18 2008

Application
Western blot
Sample
Rice Cell lysate - whole cell (THP1 cells)
Specification
THP1 cells
Blocking step
Milk as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

Submitted Nov 14 2005

Yes, the antibody is specific for holocytochrome c. It does not detect apocytochrome c. If you have any additional questions, please contact us again.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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