Anti-Homeobox protein Dlx3b antibody [Anti-Dlx3b] (ab174436)

Overview

  • Product name
    Anti-Homeobox protein Dlx3b antibody [Anti-Dlx3b]
  • Description
    Mouse monoclonal [Anti-Dlx3b] to Homeobox protein Dlx3b
  • Tested applications
    Suitable for: IHC - Wholemountmore details
  • Species reactivity
    Reacts with: Zebrafish
  • Immunogen

    Recombinant full length protein corresponding to Zebrafish Homeobox protein Dlx3b aa 1-269.
    Sequence:

    MSGPTYDRKI PGISTDLSGS MSCHPTSKDS PTLPESSATD MGYYSSHHEY YQSPPYPQQM NSYHQFNLSG MGATPGAYPT KTEYPYNTYR QYGHFNRDLQ TPPQSAVKEE PETEVRMVNG KPKKIRKPRT IYSSYQLAAL QRRFQKAQYL ALPERAELAA QLGLTQTQVK IWFQNRRSKF KKLYKNGEVP LEHSPNASDS MACNSPPSPA VWDNNAHSSQ VNRGQIPQPP LSSTPPYMED YSNHWYQQGS HLQHPVHHPG PPQSVGAVY


    Database link: Q01702

  • General notes

    This antibody clone is manufactured by Abcam.

    Product was previously marketed under the MitoSciences sub-brand.

Properties

Applications

Our Abpromise guarantee covers the use of ab174436 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC - Wholemount 1/1000.

Target

  • Relevance
    Involved in the development of the inner ear. Expressed during gastrulation, and shortly after gastrulation is expressed by presumptive precursor cells of the olfactory placodes, overlapping subsets of cells in the auditory vesicle, cells of the median fin fold, and cells of the visceral arches and their primordia. Tissue specificity: Expressed in the developing forebrain and fins.
  • Cellular localization
    Nuclear
  • Database links
  • Alternative names
    • Distal-less homeobox protein 3b antibody
    • DLX-3 antibody
    • dlx3 antibody
    • Homeobox protein Dlx3b antibody

Images

  • ab174436 staining homeobox protein Dlx3b in 19 somite zebrafish by IHC-wholemount. The embryo was fixed with 4% PFA at 4°C over night and an antigen retrieval step was performed with Tris-HCl pH 9. Blocking of the sample was done with 10% sheep serum in PBS containing 01% Tween 20 and 0.5% Triton X, for 60 minutes at 23°C, followed by staining with ab174436 at 1/100 in blocking solution for 16h at 4°C. An alexa 488 conjugated goat anti-mouse polyclonal antibody at 1/1000 was used as the secondary antibody. Nuclei are stained in blue with DAPI. The forebrain is stained by Dlx3b (in green).

  • ab174436 staining homeobox protein Dlx3b in 19 somite zebrafish by IHC-wholemount. The embryo was fixed with 4% PFA at 4°C over night and an antigen retrieval step was performed with Tris-HCl pH 9. Blocking of the sample was done with 10% sheep serum in PBS containing 01% Tween 20 and 0.5% Triton X, for 60 minutes at 23°C, followed by staining with ab174436 at 1/1000 in blocking solution for 16h at 4°C. An alexa 488 conjugated goat anti-mouse polyclonal antibody at 1/500 was used as the secondary antibody. The otic vesicle is stained by Dlx3b (in green).

    See Abreview

References

ab174436 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (differentiated induced pluripotent stem cell)
Specification
differentiated induced pluripotent stem cell
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Jul 06 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
IHC - Wholemount
Sample
Zebrafish Embryo (Otic vesicle)
Specification
Otic vesicle
Username

Dr. Ryan Macdonald

Verified customer

Submitted Aug 02 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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