Anti-Homeobox protein SIX6 antibody (ab64995)

Overview

  • Product nameAnti-Homeobox protein SIX6 antibody
    See all Homeobox protein SIX6 primary antibodies
  • Description
    Rabbit polyclonal to Homeobox protein SIX6
  • Specificityab64995 detects endogenous levels of total Homeobox protein SIX6.
  • Tested applicationsSuitable for: WB, ICC/IF, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide derived from internal sequence of human Homeobox protein SIX6.

  • Positive control
    • WB: Extracts from HUVEC cells and COLO205 cells. IF/ICC: HepG2 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab64995 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).
ICC/IF 1/500 - 1/1000.
ELISA 1/5000.

Target

Anti-Homeobox protein SIX6 antibody images

  • All lanes : Anti-Homeobox protein SIX6 antibody (ab64995) at 1/500 dilution

    Lane 1 : Extracts from HUVEC cells with no immunising peptide
    Lane 2 : Extracts from COLO205 cells with no immunising peptide
    Lane 3 : Extracts from COLO205 cells with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 28 kDa
    Observed band size : 28 kDa
  • Immunofluorescence analysis of HepG2 cells, using Homeobox protein SIX6 antibody (ab64995) at 1/500 dilution, in the presence (right panel) and absence (left panel) of immunising peptide.

References for Anti-Homeobox protein SIX6 antibody (ab64995)

ab64995 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Frozen sections)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Sample Zebrafish Tissue sections (retina)
Specification retina
Permeabilization Yes - triton X
Fixative Paraformaldehyde
Username

Dr. Ryan MacDonald

Verified customer

Submitted May 22 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"