Recombinant
RabMAb

Anti-Homer1 antibody [EPR15309] (ab184955)

Overview

  • Product name
    Anti-Homer1 antibody [EPR15309]
    See all Homer1 primary antibodies
  • Description
    Rabbit Monoclonal [EPR15309] to Homer1
  • Tested applications
    Suitable for: IP, WB, IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human Homer1 aa 100-250. The exact sequence is proprietary.
    Database link: Q86YM7

  • Positive control
    • HepG2, human fetal brain lysates, human gliocytoma tissue,
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab184955 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/60 - 1/80.
WB 1/1000 - 1/10000. Predicted molecular weight: 40 kDa.
IHC-P 1/500.
IHC-Fr 1/500.

Target

  • Function
    Postsynaptic density scaffolding protein. Binds and cross-links cytoplasmic regions of GRM1, GRM5, ITPR1, DNM3, RYR1, RYR2, SHANK1 and SHANK3. By physically linking GRM1 and GRM5 with ER-associated ITPR1 receptors, it aids the coupling of surface receptors to intracellular calcium release. May also couple GRM1 to PI3 kinase through its interaction with AGAP2. Isoform 1 regulates the trafficking and surface expression of GRM5. Isoform 3 acts as a natural dominant negative, in dynamic competition with constitutively expressed isoform 1 to regulate synaptic metabotropic glutamate function. Isoform 3, may be involved in the structural changes that occur at synapses during long-lasting neuronal plasticity and development.
  • Sequence similarities
    Belongs to the Homer family.
    Contains 1 WH1 domain.
  • Domain
    The WH1 domain interacts with the PPXXF motif in GRM1, GRM5, RYR1, RYR2, ITPR1, SHANK 1 and SHANK3.
  • Cellular localization
    Cytoplasm. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Cell junction, synapse. Isoform 1 inhibits surface expression of GRM5 causing it to be retained in the endoplasmic reticulum.
  • Information by UniProt
  • Database links
  • Alternative names
    • HOME1_HUMAN antibody
    • Homer antibody
    • HOMER 1A antibody
    • HOMER 1B antibody
    • HOMER 1C antibody
    • Homer homolog 1 (Drosophila) antibody
    • homer homolog 1 antibody
    • Homer protein homolog 1 antibody
    • homer scaffolding protein 1 antibody
    • Homer, Drosophila, homolog of, 1 antibody
    • Homer, neuronal immediate early gene, 1 antibody
    • Homer-1 antibody
    • Homer1 antibody
    • HOMER1A antibody
    • HOMER1B antibody
    • HOMER1C antibody
    • SYN47 antibody
    • Ves 1 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded Human gliocytoma labeling Homer with ab184955 at 1/500 dilution and HRP Polymer for Rabbit IgG.  Counterstained with Hematoxylin.

  • ab184955 staining Homer1 in mouse spinal cord tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.1% PBS Triton X-100 and blocked with 1% BSA for 1 hour at 21°C. Samples were incubated with primary antibody (0.1% PBS Triton X-100 + 1% BSA) for 16 hours at 4°C. An Alexa Fluor® 488 conjugated goat anti-rabbit IgG (H+L) (1/1000) was used as the secondary antibody.

    See Abreview

  • Anti-Homer1 antibody [EPR15309] (ab184955) at 1/5000 dilution + HepG2 cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size : 40 kDa
    Additional bands at : 46 kDa. We are unsure as to the identity of these extra bands.
  • Anti-Homer1 antibody [EPR15309] (ab184955) at 1/20000 dilution + Human fetal brain lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size : 40 kDa
    Additional bands at : 46 kDa. We are unsure as to the identity of these extra bands.
  • Immunoprecipitation analysis of human fetal brain labeling Homer with ab184955 at 1/50 dilution and Anti-rabbit IgG (HRP) specific to the non reduced form of IgG at 1/1500 dilution (+) or PBS (-)

References

ab184955 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Mouse Cell lysate - whole cell (Hippocampus)
Gel Running Conditions
Reduced Denaturing (Gel 10%)
Loading amount
1 µg
Specification
Hippocampus
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jun 27 2017

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Mouse spinal cord)
Permeabilization
Yes - 0.1% PBS Triton X-100
Specification
Mouse spinal cord
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Nov 18 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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