The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/10 - 1/50. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
1/1000. Predicted molecular weight: 40 kDa.
Postsynaptic density scaffolding protein. Binds and cross-links cytoplasmic regions of GRM1, GRM5, ITPR1, DNM3, RYR1, RYR2, SHANK1 and SHANK3. By physically linking GRM1 and GRM5 with ER-associated ITPR1 receptors, it aids the coupling of surface receptors to intracellular calcium release. Isoforms can be differently regulated and may play an important role in maintaining the plasticity at glutamatergic synapses.
Belongs to the Homer family. Contains 1 WH1 domain.
The WH1 domain interacts with the PPXXF motif in GRM1, GRM5, RYR1, RYR2, ITPR1, SHANK 1 and SHANK3.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human brain tissue labeling HOMER3 with ab175852 at 1/10 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow Cytometry - Anti-HOMER3 antibody (ab175852)
Flow cytometric analysis of SK-Br-3 cells labeling HOMER3 with ab175852 at 1/10 dilution (bottom histogram) compared to negative control cells (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot - Anti-HOMER3 antibody (ab175852)
All lanes : Anti-HOMER3 antibody (ab175852) at 1/1000 dilution
Lane 1 : Non-transfected 293 cell lysate Lane 2 : 293 cell lysate transiently transfected with the HOMER3 gene