Overview

  • Product name
  • Description
    Rabbit polyclonal to HOMER3
  • Tested applications
    Suitable for: Flow Cyt, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human HOMER3 aa 287-317 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q9NSC5

  • Positive control
    • MDA-MB231 and NIH3T3 cell line lysates; Human brain tissue; SK-Br-3 cells; 293 cell lysate transiently transfected with the HOMER3 gene.

Properties

Applications

Our Abpromise guarantee covers the use of ab175852 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10 - 1/50. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P 1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000. Predicted molecular weight: 40 kDa.

Target

  • Function
    Postsynaptic density scaffolding protein. Binds and cross-links cytoplasmic regions of GRM1, GRM5, ITPR1, DNM3, RYR1, RYR2, SHANK1 and SHANK3. By physically linking GRM1 and GRM5 with ER-associated ITPR1 receptors, it aids the coupling of surface receptors to intracellular calcium release. Isoforms can be differently regulated and may play an important role in maintaining the plasticity at glutamatergic synapses.
  • Sequence similarities
    Belongs to the Homer family.
    Contains 1 WH1 domain.
  • Domain
    The WH1 domain interacts with the PPXXF motif in GRM1, GRM5, RYR1, RYR2, ITPR1, SHANK 1 and SHANK3.
  • Cellular localization
    Cytoplasm. Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Cell junction > synapse. Postsynaptic density of neuronal cells.
  • Information by UniProt
  • Database links
  • Alternative names
    • HOME3_HUMAN antibody
    • HOMER 3 antibody
    • Homer 3D protein antibody
    • Homer homolog 3 (Drosophila) antibody
    • Homer neuronal immediate early gene 3 antibody
    • Homer protein homolog 3 antibody
    • Homer-3 antibody
    • Homer3 antibody
    • VESL3 antibody
    see all

Images

  • Anti-HOMER3 antibody (ab175852) at 1/1000 dilution + MDA-MB231 cell line lysate at 35 µg

    Predicted band size : 40 kDa
  • Anti-HOMER3 antibody (ab175852) at 1/1000 dilution + NIH3T3 cell line lysate at 35 µg

    Predicted band size : 40 kDa
  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human brain tissue labeling HOMER3 with ab175852 at 1/10 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.

  • Flow cytometric analysis of SK-Br-3 cells labeling HOMER3 with ab175852 at 1/10 dilution (bottom histogram) compared to negative control cells (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

  • All lanes : Anti-HOMER3 antibody (ab175852) at 1/1000 dilution

    Lane 1 : Non-transfected 293 cell lysate
    Lane 2 : 293 cell lysate transiently transfected with the HOMER3 gene

    Lysates/proteins at 2 µg per lane.


    Predicted band size : 40 kDa

References

ab175852 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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