Overview

  • Product name
    Anti-Homocysteine antibody
    See all Homocysteine primary antibodies
  • Description
    Rabbit polyclonal to Homocysteine
  • Specificity
    Compound Crossreactivity Ratio Homocysteine G BSA 1 Cysteine G BSA 1/423 Homocysteic acid G BSA 1/100,000
  • Tested applications
    Suitable for: IHC-P, IP, WB, Dot blot, ELISA, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Mammal
  • Immunogen

    Other Immunogen Type corresponding to Homocysteine conjugated to bovine serum albumin (Glutaraldehyde).

Properties

Applications

Our Abpromise guarantee covers the use of ab15154 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration.
Dot blot 1/10000.
ELISA Use at an assay dependent concentration.
IHC-FoFr Use at an assay dependent concentration. PubMed: 22419888

Target

  • Relevance
    Homocysteine is a chemical compound with the formula HSCH2CH2CH(NH2)CO2H. It is a homologue of the naturally-occurring amino acid cysteine, differing in that its side-chain contains an additional methylene (-CH2-) group before the thiol (-SH) group. Alternatively, Homocysteine can be derived from methionine by removing the latter's terminal C methyl group. Elevations of Homocysteine also occur in the rare hereditary disease homocystinuria and in methylene-tetrahydrofolate-reductase deficiency. The latter is quite common and usually goes unnoticed, although there are reports that thrombosis and cardiovascular disease occurs more often in people with elevated Homocysteine.
  • Alternative names
    • (2S) 2 amino 4 sulfanyl butanoic acid antibody

References for Anti-Homocysteine antibody (ab15154)

This product has been referenced in:
  • Qipshidze N  et al. Hydrogen sulfide mitigates cardiac remodeling during myocardial infarction via improvement of angiogenesis. Int J Biol Sci 8:430-41 (2012). IHC-FoFr ; Mouse . Read more (PubMed: 22419888) »
  • Van Campenhout A  et al. Role of homocysteine in aortic calcification and osteogenic cell differentiation. Atherosclerosis 202:557-66 (2009). Dot blot . Read more (PubMed: 18602108) »

See all 3 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 3% hydrogen peroxide
Sample
Mouse Tissue sections (eye)
Specification
eye
Permeabilization
No
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Jun 18 2013

Thank you for clarifying this.

I am currently contacting other scientists to find out more information for you.

Thank you for your patience.

This product is now published with catalogue number ab135231. Please order the product directly from website.

I hope this information will be helpful. Should you have any other inquiry please do not hesitate to contact me.

Thank you for your email.

I have forwarded your request to datasheet team. I will let you know the catalogue number for ordering soon.

Many thanks for having patience!

Application
Immunoprecipitation
Sample
Rat Tissue lysate - whole (hypocamp rat cell)
Total protein in input
600 µg
Specification
hypocamp rat cell
Immuno-precipitation step
Protein A/G
Username

Abcam user community

Verified customer

Submitted Jun 04 2012

Thank you for contacting us.

I can confirm that we can provide the peptide you have requested. The size is 1mg and the price would be ˜365£ for 1 vial. Could you please let me know if you are interested in buying this? I will then be a...

Read More
Application
Western blot
Sample
Rat Tissue lysate - whole (hypocamp cell)
Loading amount
20 µg
Specification
hypocamp cell
Gel Running Conditions
Reduced Denaturing (12)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted May 29 2012

Thank you very much for your interest in ab15154.

To our knowledge,ab15154 has not been tested in WB and we do not have any data this antibody works in WB yet.

Therefore, I can offer a discount off a future purchase if you buyab15...

Read More

Thank you for your phone call.

Below please find the information on how the testing discount program works. I have selected ab15154 as an example.

Thank you very much for your interest in ab15154.

To our knowledge, ab1515...

Read More

Thank you for getting back to me. In response to your customer; I am sorry for the confusion. I do not know where the peptide sequence that your customer cites was sourced. I can confirm that this antibody was raised against homocysteine-glutar...

Read More

1-10 of 11 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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