Homologous Recombination Panel (H2A.X, gamma H2A.X phospho S139, RAD51, RAD54) (ab115487)

Overview

  • Product nameHomologous Recombination Panel (H2A.X, gamma H2A.X phospho S139, RAD51, RAD54)
  • Product overviewab115487 is a Homologous Recombination Panel designed for the detection and characterization of key proteins involved in DNA double strand break (DSB) repair by homologous recombination (HR). Histone H2A.X is phosphorylated on S139, termed gamma H2A.X, at sites of DNA damage. DNA DSB repair by HR requires the RAD51 recombinase and the RAD54 protein.
  • Components

      Homologous Recombination Panel (H2A.X, gamma H2A.X phospho S139, RAD51, RAD54) images

      • All lanes : Anti-gamma H2A.X (phospho S139) antibody - ChIP Grade (ab2893) at 1/500 dilution

        Lane 1 : Control HeLa cell histone preparation
        Lane 2 : Colcemid treated HeLa histone preparation
        Lane 3 : Control HeLa cell histone preparation with Human gamma H2A.X (phospho S139) peptide (ab15645) at 1 µg
        Lane 4 : Colcemid treated HeLa histone preparation with Human gamma H2A.X (phospho S139) peptide (ab15645) at 1 µg
        Lane 5 : Control HeLa cell histone preparation with Human gamma H2A.X (unmodified ) peptide (ab15646) at 1 µg
        Lane 6 : Colcemid treated HeLa histone preparation with Human gamma H2A.X (unmodified ) peptide (ab15646) at 1 µg

        Secondary
        Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution
      • All lanes : Anti-Rad51 antibody (ab84304) at 1 µg/ml

        Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
        Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
        Lane 3 : Testis (Human) Tissue Lysate - adult normal tissue (ab30257)

        Lysates/proteins at 10 µg per lane.

        Secondary
        Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
        developed using the ECL technique

        Performed under reducing conditions.

        Exposure time : 20 minutes
      • Lane 1 :
        Lanes 2 - 6 : Anti-RAD54 antibody (ab10705) at 1/500 dilution

        Lane 1 : Marker
        Lane 2 : HeLa nuclear cell lysate
        Lane 3 : HeLa cell lysate
        Lane 4 : A432 cell lysate
        Lane 5 : MCF7 cell lysate
        Lane 6 : Hek 293 cell lysate

        Secondary
        Goat Anti-Rabbit IgG H&L (HRP) (ab6721)
      • Lanes 1 & 3 : Anti-Histone H2A.X antibody (ab10475) at 1/500 dilution
        Lane 2 : Anti-Histone H2A.X antibody (ab10475) at 1/1000 dilution

        Lane 1 : Calf thymus histone lysate
        Lane 2 : Calf thymus histone lysate
        Lane 3 : Calf thymus histone lysate with Human Histone H2A.X (unmodified ) peptide (ab15020) at 1 µg

        Secondary
        Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution

        Performed under reducing conditions.

        Exposure time : 30 minutes

      References for Homologous Recombination Panel (H2A.X, gamma H2A.X phospho S139, RAD51, RAD54) (ab115487)

      This product has been referenced in:
      • Stimpson KM  et al. Telomere disruption results in non-random formation of de novo dicentric chromosomes involving acrocentric human chromosomes. PLoS Genet 6: (2010). Read more (PubMed: 20711355) »
      • Zhu H  et al. Involvement of Caveolin-1 in repair of DNA damage through both homologous recombination and non-homologous end joining. PLoS One 5:e12055 (2010). Read more (PubMed: 20700465) »

      See all 2 Publications for this product

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"