Overview

  • Product nameAnti-HOOK1 antibody
    See all HOOK1 primary antibodies
  • Description
    Mouse monoclonal to HOOK1
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Cow, Dog, Chimpanzee, Rhesus monkey
  • Immunogen

    Recombinant fragment: MLLRKQLAEK ERRIEILESE CKVAKFRDYE EKLIVSAWYN KSLAFQKLGM ESRLVSGGGA CSDTGACTPA RSFLAQQRHI TNTRRNLSVK VPATTSD, corresponding to amino acids 632-728 of Human HOOK1 (NP_056972) with tag.

  • Positive control
    • MCF-7 cell lysate; Recombinant tagged human HOOK1 fragment.

Properties

Applications

Our Abpromise guarantee covers the use of ab77422 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 85 kDa.
ELISA Use at an assay dependent dilution.

Target

  • FunctionRequired for spermatid differentiation. Probably involved in the positioning of the microtubules of the manchette and the flagellum in relation to the membrane skeleton (By similarity). Component of the FTS/Hook/FHIP complex (FHF complex). The FHF complex may function to promote vesicle trafficking and/or fusion via the homotypic vesicular protein sorting complex (the HOPS complex).
  • Sequence similaritiesBelongs to the hook family.
  • Cellular localizationCytoplasm > cytoskeleton. Cytoplasm. Localizes to the spermatid manchette during spermiogenesis but is not present in mature spermatozoa (By similarity). Localizes to punctate cytoplasmic foci which do not appear to overlap with early or late endosomes, the endoplasmic reticulum, the Golgi complex, multivesicular bodies (MVBs), lysosomes, or mitochondria. Often found in close association with microtubules.
  • Information by UniProt
  • Database links
  • Alternative names
    • A930033L17Rik antibody
    • Abnormal spermatozoon head shape antibody
    • azh antibody
    • h-hook1 antibody
    • hHK1 antibody
    • HK1 antibody
    • HOOK 1 antibody
    • Hook homolog 1 (Drosophila) antibody
    • Hook1 antibody
    • HOOK1_HUMAN antibody
    • MGC10642 antibody
    • OTTHUMP00000010548 antibody
    • OTTMUSP00000008480 antibody
    • Protein Hook homolog 1 antibody
    • RP23-80B16.4 antibody
    see all

Anti-HOOK1 antibody images

  • Anti-HOOK1 antibody (ab77422) at 1 µg/ml + Recombinant tagged human HOOK1 fragment at 0.2 µg

    Secondary
    Goat anti-Mouse IgG (H&L)-HRP at 1/5000 dilution

    Predicted band size : 85 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
  • Anti-HOOK1 antibody (ab77422) at 1 µg/ml + MCF-7 cell lysate at 25 µg

    Secondary
    Goat anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

    Predicted band size : 85 kDa
    Observed band size : 90 kDa (why is the actual band size different from the predicted?)

References for Anti-HOOK1 antibody (ab77422)

ab77422 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"