Overview

  • Product nameAnti-HOOK3 antibody
    See all HOOK3 primary antibodies
  • Description
    Mouse monoclonal to HOOK3
  • Tested applicationsSuitable for: WB, ELISA, IHC-P, Sandwich ELISAmore details
  • Species reactivity
    Reacts with: Human, Recombinant Fragment
  • Immunogen

    Recombinant fragment corresponding to amino acids 622-719 of Human HOOK3, NP_115786, with proprietary tag.

  • Positive control
    • Recombinant Protein, HOOK3 transfected lysate, Human placenta

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab118091 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 83 kDa.
ELISA Use at an assay dependent concentration.
IHC-P Use a concentration of 3 µg/ml.
Sandwich ELISA Use a concentration of 10 µg/ml.

Target

  • FunctionProbably serves as a target for the spiC protein from Salmonella typhimurium, which inactivates it, leading to a strong alteration in cellular trafficking (By similarity). Component of the FTS/Hook/FHIP complex (FHF complex). The FHF complex may function to promote vesicle trafficking and/or fusion via the homotypic vesicular protein sorting complex (the HOPS complex). May regulate clearance of endocytosed receptors such as MSR1. Participates in defining the architecture and localization of the Golgi complex.
  • Sequence similaritiesBelongs to the hook family.
  • Cellular localizationCytoplasm > cytoskeleton. Golgi apparatus. Enriched at the cis-face of the Golgi complex (By similarity). Localizes to microtubule asters in prophase.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ31058 antibody
    • h-hook3 antibody
    • hHK3 antibody
    • HK3 antibody
    • hook3 antibody
    • HOOK3_HUMAN antibody
    • OTTHUMP00000229200 antibody
    • Protein Hook homolog 3 antibody
    see all

Anti-HOOK3 antibody images

  • Anti-HOOK3 antibody (ab118091) at 1 µg/ml + Recombinant Protein at 0.1 µg
    Developed using the ECL technique

    Predicted band size : 83 kDa
  • All lanes : Anti-HOOK3 antibody (ab118091) at 1 µg/ml

    Lane 1 : HOOK3 transfected lysate
    Lane 2 : Non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Developed using the ECL technique

    Predicted band size : 83 kDa
  • Immunoperoxidase of ab118091 to HOOK3 on formalin-fixed paraffin-embedded Human placenta. [antibody concentration 3 µg/ml]
  • Detection limit for recombinant proprietary tagged HOOK3 is 1 ng/ml as a capture antibody. Antibody concentration : 10 µg/ml.

References for Anti-HOOK3 antibody (ab118091)

ab118091 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"