Anti-HOPX antibody (ab106251)

Overview

  • Product nameAnti-HOPX antibody
    See all HOPX primary antibodies
  • Description
    Rabbit polyclonal to HOPX
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Pig
  • Immunogen

    Recombinant full length protein: MSAETASGPT EDQVEILEYN FNKVDKHPDS TTLCLIAAEA GLSEEETQKW FKQRLAKWRR SEGLPSECRS VTD, corresponding to amino acids 1-73 of Human HOPX (BC014225).

  • Positive control
    • Human fetal heart tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab106251 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 8 kDa (predicted molecular weight: 8 kDa).
IHC-P Use at an assay dependent concentration.

Target

  • FunctionAtypical homeodomain protein which does not bind DNA and is required to modulate cardiac growth and development. Acts via its interaction with SRF, thereby modulating the expression of SRF-dependent cardiac-specific genes and cardiac development. Prevents SRF-dependent transcription either by inhibiting SRF binding to DNA or by recruiting histone deacetylase (HDAC) proteins that prevent transcription by SRF. Overexpression causes cardiac hypertrophy (By similarity). May act as a tumor suppressor.
  • Tissue specificityWidely expressed. Expressed in the heart, brain, placenta, lung, skeletal and smooth muscles, uterus, urinary bladder, kidney and spleen. Down-regulated in some types of cancer such as lung cancer, choriocarcinoma, head and neck squamous cell carcinoma and oral squamous cell carcinoma.
  • Sequence similaritiesContains 1 homeobox DNA-binding domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • FormThere are 2 isoforms produced by alternative splicing.
  • Alternative names
    • CAMEO antibody
    • heart odd homeobox 1 protein antibody
    • HOD antibody
    • Homeodomain-only protein antibody
    • HOP antibody
    • HOP homeobox antibody
    • HOP_HUMAN antibody
    • HOPX antibody
    • LAGY antibody
    • Lung cancer-associated Y protein antibody
    • NECC1 antibody
    • Not expressed in choriocarcinoma clone 1 antibody
    • Not expressed in choriocarcinoma protein 1 antibody
    • OB1 antibody
    • odd homeobox 1 protein antibody
    • Odd homeobox protein 1 antibody
    • OTTHUMP00000158970 antibody
    • SMAP31 antibody
    • TOTO antibody
    see all

Anti-HOPX antibody images

  • Anti-HOPX antibody (ab106251) at 1/500 dilution + Human fetal heart tissue lysate

    Predicted band size : 8 kDa

References for Anti-HOPX antibody (ab106251)

ab106251 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Brain)
Permeabilization Yes - Triton X-100
Specification Brain
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative Formaldehyde
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Verified customer

Submitted Jul 20 2016

Thank you for your telephone enquiry this afternoon. I am sorry to hear you have experienced difficulty obtaining satisfactory results from this antibody in western blotting.The details provided enable us to closely monitor the quality of our products....

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"