The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 35 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
FunctionInvolved in meiotic progression.
Tissue specificityExpressed in testis, liver and brain, and at lower levels in kidney.
Sequence similaritiesContains 1 HORMA domain.
Cellular localizationNucleus. Chromosome. Preferentially localizes to unsynapsed or desynapsed chromosomal regions during the male and female prophase I stage of meiosis. TRIP13 is required for depletion from synapsed chromosomes.