1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
FunctionIn adipose tissue and heart, it primarily hydrolyzes stored triglycerides to free fatty acids, while in steroidogenic tissues, it principally converts cholesteryl esters to free cholesterol for steroid hormone production.
Staining of HSL/LIPE in formalin fixed and paraffin embedded Human testis tissue labelled with ab103281 at a 1/50 dilutuion, which was peroxidase conjugated to the secondary antibody followed by DAB staining.
References for Anti-Hormone sensitive lipase antibody (ab103281)
has not yet been referenced specifically in any publications.
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