Overview

  • Product nameAnti-Hormone sensitive lipase antibody
    See all Hormone sensitive lipase primary antibodies
  • Description
    Rabbit polyclonal to Hormone sensitive lipase
  • Tested applicationsSuitable for: IHC-P, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic 14 amino acid peptide from near the carboxy terminus of human HSL/LIPE

  • Positive control
    • Human lymph node tissue lysate.

Applications

Our Abpromise guarantee covers the use of ab45422 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 2.5 µg/ml.
ICC/IF Use a concentration of 20 µg/ml.
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 117 kDa).

Target

Anti-Hormone sensitive lipase antibody images

  • ab45422 staining Hormone sensitive lipase in the HeLa cell line from Human cervical cancer by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and permeabilized with 0.5% Triton X-100 in PBS. Samples were incubated with primary antibody (1/200 in PBS) for 16 hour at 22°C. ab150081 an Alexa Fluor®488-conjugated Goat anti-rabbit IgG H&L (1/200) polyclonal was used as the secondary antibody. Nuclei were stained with DAPI.MeOH-fixed samples were also evaluated and failed to produce the expected staining pattern.

    See Abreview

  • Lane 1 : Anti-Hormone sensitive lipase antibody (ab45422) at 0.5 µg/ml
    Lane 2 : Anti-Hormone sensitive lipase antibody (ab45422) at 1 µg/ml

    Lane 1 : Human lymph node tissue lysate
    Lane 2 : Human lymph node tissue lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 117 kDa
    Observed band size : 90 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 38 kDa,86 kDa,88 kDa. We are unsure as to the identity of these extra bands.
  • Immunofluorescence of Lipe in Human Lymph Node cells using ab45422 at 20 ug/ml.

References for Anti-Hormone sensitive lipase antibody (ab45422)

This product has been referenced in:
  • Jiao J  et al. Chronic leucine supplementation improves lipid metabolism in C57BL/6J mice fed with a high-fat/cholesterol diet. Food Nutr Res 60:31304 (2016). WB ; Mouse . Read more (PubMed: 27616737) »
  • Ikoma-Seki K  et al. Role of LRP1 and ERK and cAMP Signaling Pathways in Lactoferrin-Induced Lipolysis in Mature Rat Adipocytes. PLoS One 10:e0141378 (2015). WB ; Rat . Read more (PubMed: 26506094) »

See all 8 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Permeabilization Yes - 0.5% Triton-X100 in PBS
Fixative Paraformaldehyde
Username

Dr. Kirk McManus

Verified customer

Submitted Nov 05 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"