Anti-Hormone sensitive lipase/HSL antibody (ab45422)
Key features and details
- Rabbit polyclonal to Hormone sensitive lipase/HSL
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Hormone sensitive lipase/HSL antibody
See all Hormone sensitive lipase/HSL primary antibodies -
Description
Rabbit polyclonal to Hormone sensitive lipase/HSL -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
This antibody is an immunoaffinity purified IgG -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Cholesterol Metabolism
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab45422 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use at an assay dependent concentration.
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ICC/IF | (1) |
Use a concentration of 20 µg/ml.
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WB |
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 117 kDa).
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Notes |
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IHC-P
Use at an assay dependent concentration. |
ICC/IF
Use a concentration of 20 µg/ml. |
WB
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 117 kDa). |
Target
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Function
In adipose tissue and heart, it primarily hydrolyzes stored triglycerides to free fatty acids, while in steroidogenic tissues, it principally converts cholesteryl esters to free cholesterol for steroid hormone production. -
Pathway
Glycerolipid metabolism; triacylglycerol degradation. -
Sequence similarities
Belongs to the 'GDXG' lipolytic enzyme family. -
Cellular localization
Cell membrane. Membrane > caveola. Cytoplasm > cytosol. Found in the high-density caveolae. Translocates to the cytoplasm from the caveolae upon insulin stimulation. - Information by UniProt
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Database links
- Entrez Gene: 3991 Human
- Entrez Gene: 16890 Mouse
- Entrez Gene: 25330 Rat
- Omim: 151750 Human
- SwissProt: Q05469 Human
- SwissProt: Q6NSL7 Human
- SwissProt: P54310 Mouse
- SwissProt: P15304 Rat
see all -
Alternative names
- Hormone sensitive lipase antibody
- Hormone sensitive lipase testicular isoform antibody
- Hormone-sensitive lipase antibody
see all
Images
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ab45422 staining Hormone sensitive lipase/HSL in the HeLa cell line from Human cervical cancer by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and permeabilized with 0.5% Triton X-100 in PBS. Samples were incubated with primary antibody (1/200 in PBS) for 16 hour at 22°C. ab150081 an Alexa Fluor®488-conjugated Goat anti-rabbit IgG H&L (1/200) polyclonal was used as the secondary antibody. Nuclei were stained with DAPI.MeOH-fixed samples were also evaluated and failed to produce the expected staining pattern.
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Lane 1 : Anti-Hormone sensitive lipase/HSL antibody (ab45422) at 0.5 µg/ml
Lane 2 : Anti-Hormone sensitive lipase/HSL antibody (ab45422) at 1 µg/ml
All lanes : Human lymph node tissue lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 117 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?
Additional bands at: 38 kDa, 86 kDa, 88 kDa. We are unsure as to the identity of these extra bands. -
Immunofluorescence of Lipe in Human Lymph Node cells using ab45422 at 20 ug/ml.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (45)
ab45422 has been referenced in 45 publications.
- Zhong R et al. Nomegestrol acetate ameliorated adipose atrophy in a rat model of cisplatin‑induced cachexia. Exp Ther Med 25:24 (2023). PubMed: 36561625
- Yang X et al. Targeting adipocytic discoidin domain receptor 2 impedes fat gain while increasing bone mass. Cell Death Differ 29:737-749 (2022). PubMed: 34645939
- Dong J et al. Increased adipose tissue lipolysis in dairy cows with fatty liver is associated with enhanced autophagy activity. J Dairy Sci 105:1731-1742 (2022). PubMed: 34998548
- Plaza A et al. Cholecystokinin promotes functional expression of the aquaglycerol channel aquaporin 7 in adipocytes. Br J Pharmacol 179:4092-4106 (2022). PubMed: 35366004
- Ferrando M et al. Renal peritumoral adipose tissue undergoes a browning process and stimulates the expression of epithelial-mesenchymal transition markers in human renal cells. Sci Rep 12:8687 (2022). PubMed: 35606546