Anti-Hormone sensitive lipase antibody (ab73736)

Overview

  • Product nameAnti-Hormone sensitive lipase antibody
    See all Hormone sensitive lipase primary antibodies
  • Description
    Rabbit polyclonal to Hormone sensitive lipase
  • SpecificityThis antibody detects endogenous levels of total HSL/LIPE protein.
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    A synthesized non-phosphopeptide derived from human HSL/LIPE around the phosphorylation site of serine 552 (R-R-SP-V-S).

  • Positive control
    • Human tonsil tissue and extracts from Jurkat cells and MCF-7 cells.

Applications

Our Abpromise guarantee covers the use of ab73736 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 117 kDa (predicted molecular weight: 117 kDa).
ELISA 1/10000.
IHC-P Use at an assay dependent concentration.

Target

Anti-Hormone sensitive lipase antibody images

  • All lanes : Anti-Hormone sensitive lipase antibody (ab73736) at 1/500 dilution

    Lane 1 : Extracts from Jurkat cells
    Lane 2 : Extracts from MCF-7 cells
    Lane 3 : Extracts from Jurkat cells plus 10µg immunizing peptide

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 117 kDa
    Observed band size : 117 kDa
  • Immunohistochemistry analysis of paraffin-embedded human tonsil tissue using ab73736 at a 1:50 dilution.

    Left image un-treated.

    Right image treated with immunizing peptide.

References for Anti-Hormone sensitive lipase antibody (ab73736)

This product has been referenced in:
  • Greineisen WE  et al. Insulin-containing lipogenic stimuli suppress mast cell degranulation potential and up-regulate lipid body biogenesis and eicosanoid secretion in a PPAR?-independent manner. J Leukoc Biol 92:653-65 (2012). Read more (PubMed: 22706316) »

See 1 Publication for this product

Product Wall

ab63492 and ab73736 are not phospho-specific antibodies, so they should not bind to phosphorylated HSL. These antibodies are first affinity-purified from rabbit serum by affinity chromatography using epitope-specific phosphopeptide. The flowthrough was...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"